Team:KAIT Japan/Notebook

From 2014.igem.org

(Difference between revisions)
Line 66: Line 66:
:<big>'''Date:8/26'''</big> Insert gene into TAvectar and Transformation
:<big>'''Date:8/26'''</big> Insert gene into TAvectar and Transformation
:<big>'''Date:8/26'''</big> Blue white Selection and Electrophoresis(8/25 Restriction) and DNA Extraction
:<big>'''Date:8/26'''</big> Blue white Selection and Electrophoresis(8/25 Restriction) and DNA Extraction
 +
:<big>'''Date:8/27'''</big> Electrophoresed the DNA that We did refinement on August 26 to confirmed it
 +
:<big>'''Date:
 +
 +
 +
 +
 +
<br>
<br>
<br>
<br>

Revision as of 04:57, 19 September 2014

ロゴ2.png

KAIT Japan2013 Kanako.png

KAIT Japan 2014 iGEMRogo.png
New home t.jpg

Home

New Team1.jpg

Team

New 考える人.jpg

Project

New 歯車.jpg

Parts

New １１１１１１１１１１１１１１１.jpg

Protocol

Ff.jpg

Notebook

New 結果.jpg

Results

New new 危険.jpg

Safety

New new ひと.jpg

Human Practice


Notebook

We made an experiment every day. It was process at trial and error.

Basic task

Date:8/18 Miniprep



Creating parts of HlyA and GFP

Date:8/22 The refinement of DNA and PCR and Electrophoresis
Date:8/25 PCR and Electrophoresis and Restriction
Date:8/26 Insert gene into TAvectar and Transformation
Date:8/26 Blue white Selection and Electrophoresis(8/25 Restriction) and DNA Extraction
Date:8/27 Electrophoresed the DNA that We did refinement on August 26 to confirmed it
Date:





Creating parts of STAT3

Date:8/19 PCR(8/18 Miniprep) and Electrophoresis
Date:8/20~8/22 PCR(Lowered 2℃ from Tm value.) and Electrophoresis
Date:8/25 PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)
Date:8/26 PCR and Electrophoresis(Using a mutation primer.)
Date:8/27 First Variation introduction (with Prime STAR Max)
Date:9/1
Date:9/2
Date:9/8 PCR and Electrophoresis
Date:9/9 Sequence(to To confirm whether variation happened in DNA of STAT3 )
Date:9/10~9/12 PCR and Electrophoresis



Creating parts of IL-10α,IL-10β

Date:8/19 PCR(8/18 Miniprep) and Electrophoresis
Date:8/20~8/22 PCR(Lowered 2℃ from Tm value.) and Electrophoresis
Date:8/25 PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)
Date:8/26 PCR and Electrophoresis(Using a mutation primer.)
Date:8/27 First Variation introduction (with Prime STAR Max)
Date:9/1
Date:9/2
Date:9/8
Date:9/9 Sequence(to To confirm whether variation happened in DNA of IL-10α,IL-10β)
Date:9/10~9/12


Retrieved from "http://2014.igem.org/Team:KAIT_Japan/Notebook"