Team:CSU Fort Collins

From 2014.igem.org

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          <h1>Welcome to CSU's 2014 iGEM Wiki</h1>
 
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Approximately 3 billion gallons per year of used frying oil are produced in the U.S. alone. While some recycling efforts are put forth to turn used frying oil into biodiesel and some companies are working on more effective ways to recycle the frying oil, the iGEM team at Colorado State University is working to turn spent frying oil into a high value product. There are four major components to this year’s project including; the breakdown of frying oil, a biosensor for detecting the breakdown of frying oil, production of a high value product, and a kill switch to kill the bacteria if they were to be released into the environment. Our team aims to put each of these components into <i>Escherichia coli</i>. 
 
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In order to detect that the process is working we have developed a biosensor that will detect the breakdown of Acyl-CoA, which is a byproduct of the breakdown pathway of lipids in E. coli. Our biosensor is being constructed by inserting a novel promoter in front of DNA expressing a Green Fluorescent Protein (GFP). This promoter is activated by Acyl-CoA. This will result in <i>E. coli</i> expressing GFP when successful breakdown is occurring.
 
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In order to break down the frying oil we will be taking advantage of the cell’s natural ability to break down fatty acids for use in the Kreb’s cycle. We will be up-regulating the limiting enzymes that aid in the breakdown of fatty acids which will get the cell to produce more Acetyl-CoA.
 
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This Acetyl-CoA can then be used in the mevalonate pathway to produce terpenoids. This pathway is common to most plants as well as yeast. We will be taking the mevalonate pathway genes from yeast and constructing two operons in order to produce the terpenoid intermediates isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). While both IPP and DMAPP are both naturally produced in <i>E. coli</i> through the non-mevalonate pathway, the mevalonate pathway provides an efficient route from acetyl-CoA to terpenoid production than the alternative. Our high-value product will be a terpenoid that has yet to be determined.
 
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For our kill switch will be using a gene named Killer Red which is a mutant of hydrozoan chromoprotein anm2CP from Anthomedusae sp. DC-2005. Our team plans to insert this gene into our construct and by doing so, it will provide a fail-safe in the unlikely event of our <i>E. coli</i> escaping into the environment. The Killer Red gene has a repressible promoter in which tryptophan acts as the repressor. There will be tryptophan present while the cell is used to break down the frying oil, but is not highly present in the environment. Without the presence of tryptophan the Killer Red gene will be activated and white light will produce reactive oxygen species within the cell, causing cell death.
 
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  <li class='active'><a href='/Team:CSU_Fort_Collins'><span>Home</span></a></li>
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  <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Project/'><span>Project</span></a>
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      <ul>
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        <li><a href='/Team:CSU_Fort_Collins/Biosensor/'><span>Biosensor</span></a></li>
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        <li><a href='/Team:CSU_Fort_Collins/Breakdown/'><span>Breakdown</span></a></li>
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        <li><a href='/Team:CSU_Fort_Collins/HVP/'><span>High-Value Product</span></a></li>
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        <li><a href='/Team:CSU_Fort_Collins/Members/'><span>Members</span></a></li>
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  <li class='has-sub'><a href='Team:CSU_Fort_Collins/Notebook/'><span>Notebook</span></a>
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      <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Protocols'><span>Protocols</span></a>
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          <li class='last'><a href="#"><span>Protocol 1</span></a></li>
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      <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/DailyNotes'><span>Daily Notes</span></a>
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        <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/'><span>Biosensor</span></a>
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            <ul>
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              <li><a href="#"><span>June</span></a></li>
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              <li><a href="#"><span>July</span></a></li>
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              <li><a href='#'><span>August</span></a></li>
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              <li class='last'><a href="#"><span>September</span></a></li>
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        <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/'><span>Breakdown</span></a>
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              <li><a href="#"><span>June</span></a></li>
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              <li><a href="#"><span>July</span></a></li>
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              <li><a href='#'><span>August</span></a></li>
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              <li class='last'><a href="#"><span>September</span></a></li>
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            </ul>
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        <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/HVP/'><span>High-Value Product</span></a>
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            <ul>
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              <li><a href="#"><span>June</span></a></li>
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              <li><a href="#"><span>July</span></a></li>
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              <li><a href='#'><span>August</span></a></li>
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              <li class='last'><a href="#"><span>September</span></a></li>
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            </ul>
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        </li>
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        <li li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/KillSwitch/'><span>Kill Switch</span></a>
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            <ul>
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              <li><a href="#"><span>June</span></a></li>
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              <li><a href="#"><span>July</span></a></li>
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              <li><a href='#'><span>August</span></a></li>
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              <li class='last'><a href="#"><span>September</span></a></li>
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  <li class='has-sub'><a href='/Team:CSU_Fort_Collins/HumPrac/'><span>Human Practices</span></a>
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        <li><a href='/Team:CSU_Fort_Collins/Collab/'><span>Collaboration</span></a></li>
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        <li><a href='/Team:CSU_Fort_Collins/Outreach/'><span>Outreach</span></a></li>
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        <li class='last'><a href='/Team:CSU_Fort_Collins/Ethics'><span>Ethics Miniseries</span></a></li>
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  <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Achievements/'><span>Achievements</span></a>
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        <li><a href='/Team:CSU_Fort_Collins/Parts/'><span>Parts</span></a></li>
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        <li class='last'><a href='/Team:CSU_Fort_Collins/Judging/'><span>Judging</span></a></li>
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  <li><a href='/Team:CSU_Fort_Collins/Safety/'><span>Safety</span></a></li>
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  <li class='last'><a href='https://2014.igem.org/Main_Page'><img src='http://oi62.tinypic.com/11rxaxj.jpg' class='logo'/></a></li>
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    <h1>Our Team</h1>
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    <div class="col" data-name="adriana-collings">
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          <img src="https://static.igem.org/mediawiki/2014/thumb/6/6d/CSU_tm1.jpg/600px-CSU_tm1.jpg" class="pic"/><br>
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          Adriana Collings<br>
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          <a href="mailto:adriana.collings@rams.colostate.edu"><img src="https://static.igem.org/mediawiki/2014/thumb/1/13/CSU_EmailC.png/600px-CSU_EmailC.png" class="icon"/></a>
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          <a href="http://www.linkedin.com/pub/adriana-collings/9a/b1b/5a2"><img src="https://static.igem.org/mediawiki/2014/thumb/e/e2/CSU_LinkC.png/600px-CSU_LinkC.png" class="icon"/></a>
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    </div>
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    <div class="col" data-name="anthony-roulier">
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          <img src="https://static.igem.org/mediawiki/2014/0/0b/CSU_tm3.jpg" class="pic"/><br>
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          Anthony Roulier<br>
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          <a href="mailto:aroulier@rams.colostate.edu"><img src="https://static.igem.org/mediawiki/2014/thumb/1/13/CSU_EmailC.png/600px-CSU_EmailC.png" class="icon"/></a>
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    </div>
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    <div class="col" data-name="chauncy-hinshaw">
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          <img src="https://static.igem.org/mediawiki/2014/thumb/9/9b/CSU_tm2.jpg/600px-CSU_tm2.jpg" class="pic"/><br>
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          Chauncy Hinshaw<br>
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          <a href="mailto:chauncyh@rams.colostate.edu"><img src="https://static.igem.org/mediawiki/2014/thumb/1/13/CSU_EmailC.png/600px-CSU_EmailC.png" class="icon"/></a>
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    <div class="bio" id="adriana-collings">
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          <b>Major:</b> Microbiology<br>
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          <b>Semester:</b> 4<br>
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          <b>iGEM Topic:</b> High-Value Product<br>
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          <b>Other Positions:</b> Lab Assistant (Tuberculosis research), President, Synthetic Biology Club<br>
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          <b>Interests:</b> Molecular Biology, Immunology, Bacteriology<br>
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    </div>
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    <div class="bio" id="anthony-roulier">
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          <b>Major:</b> Chemical/Biological Engineering<br>
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          <b>Semester:</b> 4<br>
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          <b>iGEM Topic:</b> Breakdown<br>
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          <b>Other Positions:</b> Member, AICHE<br>
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          <b>Interests:</b> Fermentation, Food Microbiology<br>
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    </div>
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    <div class="bio" id="chauncy-hinshaw">
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          <b>Major:</b> Microbiology<br>
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          <b>Semester:</b> 4<br>
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          <b>iGEM Topic:</b> Breakdown<br>
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          <b>Other Positions:</b> Lab Assistant (Tuberculosis research); President, Microbiology Student Association; Treasurer, College of Veterinary
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          Medicine and Biosciences College Council<br>
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          <b>Interests:</b> Epidemiology, Public Health Education, Gene Therapy<br>
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    <div class="col" data-name="josiah-racchini">
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        <img src="https://static.igem.org/mediawiki/2014/thumb/0/06/CSU_tm6.jpg/600px-CSU_tm6.jpg" class="pic"/><br>
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        Josiah Racchini<br>
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        <a href="mailto:racchini@rams.colostate.edu"><img src="https://static.igem.org/mediawiki/2014/thumb/1/13/CSU_EmailC.png/600px-CSU_EmailC.png" class="icon"/></a>
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        <a href="http://www.linkedin.com/pub/josiah-racchini/72/1bb/698"><img src="https://static.igem.org/mediawiki/2014/thumb/e/e2/CSU_LinkC.png/600px-CSU_LinkC.png" class="icon"/></a>
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    </div>
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    <div class="col" data-name="krista-henderson">
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          <img src="https://static.igem.org/mediawiki/2014/thumb/0/04/CSU_tm4.jpg/600px-CSU_tm4.jpg" class="pic"/><br>
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          Krista Henderson<br>
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          <a href="mailto:kristalh@rams.colostate.edu"><img src="https://static.igem.org/mediawiki/2014/thumb/1/13/CSU_EmailC.png/600px-CSU_EmailC.png" class="icon"/></a>
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          <a href="http://www.linkedin.com/in/hendersonkrista"><img src='https://static.igem.org/mediawiki/2014/thumb/e/e2/CSU_LinkC.png/600px-CSU_LinkC.png' class='icon'/></a>
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    </div>
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    <div class="col" data-name="matt-sabel">
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          <img src="https://static.igem.org/mediawiki/2014/thumb/5/59/CSU_tm8.jpg/600px-CSU_tm8.jpg" class="pic"/><br>
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          Matt Sabel<br>
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          <a href="mailto:mesabel@rams.colostate.edu"><img src="https://static.igem.org/mediawiki/2014/thumb/1/13/CSU_EmailC.png/600px-CSU_EmailC.png" class="icon"/></a>
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    </div>
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    <div class="bio" id="josiah-racchini">
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          <b>Major:</b> Biochemistry<br>
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          <b>Semester:</b> 6<br>
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          <b>iGEM Topic:</b> Biosensor<br>
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          <b>Other Positions:</b> Lab Assistant (Tomato mosaic virus research)<br>
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          <b>Interests:</b> Epigenetics, Manufacturing<br>
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    </div>
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    <div class="bio" id="krista-henderson">
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          <b>Major:</b> Chemical/Biological Engineering<br>
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          <b>Semester:</b> 4<br>
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          <b>iGEM Topic:</b> Breakdown<br>
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          <b>Other Positions:</b> Member, CSU Marching Band<br>
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          <b>Interests:</b> Scientific Literacy, Gene Therapy<br>   
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    </div>
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    <div class="bio" id="matt-sabel">
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          <b>Major:</b> Microbiology<br>
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          <b>Semester:</b> 2<br>
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          <b>iGEM Topic:</b> Kill Switch<br>
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          <b>Other Positions:</b> IT (CSU Veterinary Teaching Hospital)<br>
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          <b>Interests:</b> Virology, Pathology<br></td>
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    </div>
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    <div class="clear"></div>
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    <div class="col" data-name="olivia-smith">
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          <img src="https://static.igem.org/mediawiki/2014/thumb/a/a0/CSU_tm9.jpg/600px-CSU_tm9.jpg" class="pic"/><br>
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          Olivia Smith<br>
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          <a href="mailto:ossmith@rams.colostate.edu"><img src="https://static.igem.org/mediawiki/2014/thumb/1/13/CSU_EmailC.png/600px-CSU_EmailC.png" class="icon"/></a>
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          <a href="http://www.linkedin.com/pub/olivia-smith/77/608/911"><img src="https://static.igem.org/mediawiki/2014/thumb/e/e2/CSU_LinkC.png/600px-CSU_LinkC.png" class="icon"/></a>
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          <a href="http://twitter.com/LunaKCSU"><img src="https://static.igem.org/mediawiki/2014/thumb/6/67/CSU_TwitterC.png/600px-CSU_TwitterC.png" class="icon"></a>
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    </div>
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    <div class="col" data-name="renee-plomondon">
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        <img src="https://static.igem.org/mediawiki/2014/thumb/b/b3/CSU_tm7.jpg/600px-CSU_tm7.jpg" class="pic"/><br>
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        Renee Plomondon<br>
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        <a href="mailto:reneeplomondon@gmail.com"><img src="https://static.igem.org/mediawiki/2014/thumb/1/13/CSU_EmailC.png/600px-CSU_EmailC.png" class="icon"/></a>
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    </div>
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    <div class="col" data-name="savannah-roemer">
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          <img src="https://encrypted-tbn1.gstatic.com/images?q=tbn:ANd9GcSRxz0Msy361i7dv4XDmzpMIsxifRk-iZSgXmynC0cgZB9mlpqqVFXeJh4" class="pic"/><br>
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          Savannah Roemer<br>
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          <a href="mailto:sroemer@rams.colostate.edu"><img src="https://static.igem.org/mediawiki/2014/thumb/1/13/CSU_EmailC.png/600px-CSU_EmailC.png" class="icon"/></a>
 +
    </div>
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    <div class="clear"></div>
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    <div class="bio" id="olivia-smith">
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          <b>Major:</b> Chemical/Biological Engineering, Biomedical Engineering<br>
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          <b>Semester:</b> 4<br>
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          <b>iGEM Topic:</b> High-Value Product<br>
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          <b>Other Positions:</b> Lab Assistant (Hepatocyte research); Underwriting Director, DJ (KCSU 90.5 FM)<br>
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          <b>Interests:</b> Tissue Engineering, Web/Graphic Design, Programming<br>
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    </div>
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    <div class="bio" id="renee-plomondon">
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          <b>Major:</b> Biochemistry<br>
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          <b>Semester:</b> 2<br>
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          <b>iGEM Topic:</b> Kill Switch<br>
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          <b>Other Positions:</b> Lab Assistant (Musculoskeletal Biomechanics research)<br>
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          <b>Interests:</b> Bionics, Biomedicine<br>
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    </div>
 +
    <div class="bio" id="savannah-roemer">
 +
          <b>Major:</b> Chemical/Biological Engineering<br>
 +
          <b>Semester:</b> 4<br>
 +
          <b>Other Positions:</b> Lab Assistant (Tuberculosis research), International Field Biology Research Assistant (Euglossine bees, entomology research)<br>
 +
          <b>Interests:</b> Biochemistry, Genetics, Horticulture<br>
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    </div>     
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    <div class="clear"></div>
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    <h1>Our Advisers</h1>
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    <div class='col' data-name='sun-jiayi'>
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          <img src='https://static.igem.org/mediawiki/2014/thumb/2/2c/CSU_tm10.jpg/600px-CSU_tm10.jpg' class='pic'/><br>
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          Sun Jiayi<br>
 +
          <a href='mailto:jiayisun@engr.colostate.edu'><img src='https://static.igem.org/mediawiki/2014/thumb/1/13/CSU_EmailC.png/600px-CSU_EmailC.png' class='icon'/></a>
 +
    </div>
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    <div class="col" data-name="ashok-prasad">
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          <img src="https://static.igem.org/mediawiki/2014/thumb/1/10/Ashok.jpg/600px-Ashok.jpg" class="pic"/><br>
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          Ashok Prasad, Ph.D.<br>
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          <a href="mailto:ashokp@engr.colostate.edu"><img src="https://static.igem.org/mediawiki/2014/thumb/1/13/CSU_EmailC.png/600px-CSU_EmailC.png" class="icon"/></a>
 +
    </div>
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    <div class="col" data-name="christie-peebles">
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          <img src="https://static.igem.org/mediawiki/2014/a/a1/Christie.jpg" class="pic"/><br>
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          Christie Peebles, Ph.D.<br>
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          <a href="mailto:cpeebles@engr.colostate.edu"><img src="https://static.igem.org/mediawiki/2014/thumb/1/13/CSU_EmailC.png/600px-CSU_EmailC.png" class="icon"/></a>
 +
          <a href='www.linkedin.com/pub/christie-peebles/80/459/b0'><img src="https://static.igem.org/mediawiki/2014/thumb/e/e2/CSU_LinkC.png/600px-CSU_LinkC.png" class="icon"/></a>
 +
    </div>
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    <div class="clear"></div>
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    <div class='bio' id='sun-jiayi'>
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          <b>Department:</b> Chemical/Biological Engineering<br>
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          <b>Research Interests:</b> Sun is a graduate student at CSU working in Dr. Peebles lab. Specifically, she works with periwinkles. She advises all of the iGEM team members in day-to-day activities.<br><br>
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    </div>
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    <div class="bio" id="ashok-prasad">
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          <b>Department:</b> Chemical/Biological Engineering<br>
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          <b>Research Interests:</b> Dr. Prasad's research is driven by concrete biological and bio-engineering questions as well as the larger questions posed by systems biology and quantitative biology. He investigates questions such as 'What does it mean to say that life is an emergent property of complex biological networks? Are there non-trivial universal principles that "living matter" obeys?' The main method his lab uses to answer these questions is creation and development of predictive biological models.<br><br>
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    </div>
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    <div class="bio" id="christie-peebles">
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          <b>Department:</b> Chemical/Biological Engineering<br>
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          <b>Research Interests:</b> Dr. Peebles' research is focused in the areas of metabolic engineering, secondary metabolism, regulatory networks, and systems biology in plants, bacteria and yeast for the production of bio-based chemicals and fuels.  More specifically, these areas can be divided into 2 main applications.  One application is the use of plant metabolic engineering to produce important pharmaceuticals and nutraceuticals.  The second is the engineering of photoautotrophs for the production of bio-based chemicals and fuels.<br><br>
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Revision as of 16:13, 18 September 2014

Safety




Our Team


Adriana Collings

Anthony Roulier

Chauncy Hinshaw
Major: Microbiology
Semester: 4
iGEM Topic: High-Value Product
Other Positions: Lab Assistant (Tuberculosis research), President, Synthetic Biology Club
Interests: Molecular Biology, Immunology, Bacteriology
Major: Chemical/Biological Engineering
Semester: 4
iGEM Topic: Breakdown
Other Positions: Member, AICHE
Interests: Fermentation, Food Microbiology
Major: Microbiology
Semester: 4
iGEM Topic: Breakdown
Other Positions: Lab Assistant (Tuberculosis research); President, Microbiology Student Association; Treasurer, College of Veterinary Medicine and Biosciences College Council
Interests: Epidemiology, Public Health Education, Gene Therapy

Josiah Racchini

Krista Henderson

Matt Sabel
Major: Biochemistry
Semester: 6
iGEM Topic: Biosensor
Other Positions: Lab Assistant (Tomato mosaic virus research)
Interests: Epigenetics, Manufacturing
Major: Chemical/Biological Engineering
Semester: 4
iGEM Topic: Breakdown
Other Positions: Member, CSU Marching Band
Interests: Scientific Literacy, Gene Therapy
Major: Microbiology
Semester: 2
iGEM Topic: Kill Switch
Other Positions: IT (CSU Veterinary Teaching Hospital)
Interests: Virology, Pathology

Olivia Smith

Renee Plomondon

Savannah Roemer
Major: Chemical/Biological Engineering, Biomedical Engineering
Semester: 4
iGEM Topic: High-Value Product
Other Positions: Lab Assistant (Hepatocyte research); Underwriting Director, DJ (KCSU 90.5 FM)
Interests: Tissue Engineering, Web/Graphic Design, Programming
Major: Biochemistry
Semester: 2
iGEM Topic: Kill Switch
Other Positions: Lab Assistant (Musculoskeletal Biomechanics research)
Interests: Bionics, Biomedicine
Major: Chemical/Biological Engineering
Semester: 4
Other Positions: Lab Assistant (Tuberculosis research), International Field Biology Research Assistant (Euglossine bees, entomology research)
Interests: Biochemistry, Genetics, Horticulture

Our Advisers


Sun Jiayi

Ashok Prasad, Ph.D.

Christie Peebles, Ph.D.
Department: Chemical/Biological Engineering
Research Interests: Sun is a graduate student at CSU working in Dr. Peebles lab. Specifically, she works with periwinkles. She advises all of the iGEM team members in day-to-day activities.

Department: Chemical/Biological Engineering
Research Interests: Dr. Prasad's research is driven by concrete biological and bio-engineering questions as well as the larger questions posed by systems biology and quantitative biology. He investigates questions such as 'What does it mean to say that life is an emergent property of complex biological networks? Are there non-trivial universal principles that "living matter" obeys?' The main method his lab uses to answer these questions is creation and development of predictive biological models.

Department: Chemical/Biological Engineering
Research Interests: Dr. Peebles' research is focused in the areas of metabolic engineering, secondary metabolism, regulatory networks, and systems biology in plants, bacteria and yeast for the production of bio-based chemicals and fuels. More specifically, these areas can be divided into 2 main applications. One application is the use of plant metabolic engineering to produce important pharmaceuticals and nutraceuticals. The second is the engineering of photoautotrophs for the production of bio-based chemicals and fuels.

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