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Team:Evry/Interlab Study/09-02-2014
From 2014.igem.org
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| - | <h2>Construction n°1: PSB1C3 with I20260</h2> | + | <br/><h2>Construction n°1: PSB1C3 with I20260</h2> |
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| + | Analyse of sequencing results. The two constructions were ok although the colony 4 had a better match. So we decided to keep this colony to perform promoter activity assays. | ||
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<h2>Constructions with other promoters</h2> | <h2>Constructions with other promoters</h2> | ||
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| - | Miniprep cultures from the 2cnd of september was purified with the NucleoSpin Plasmid kit (Macherey-Nagel). DNA was quantify with the Nanodrop 2000 | + | Miniprep cultures from the 2cnd of september was purified with the NucleoSpin Plasmid kit (Macherey-Nagel). DNA was quantify with the Nanodrop 2000. |
| + | <br/> Glycerol stock were prepared started from the 16 cultures from the 2cnd of september (750 µl of culture + 750 µL of 10% glycerol) and stored at -80°C. | ||
<span class="cd-date">Sep 02</span> | <span class="cd-date">Sep 02</span> | ||
Revision as of 18:32, 17 September 2014
Construction n°1: PSB1C3 with I20260
Analyse of sequencing results. The two constructions were ok although the colony 4 had a better match. So we decided to keep this colony to perform promoter activity assays.
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Constructions with other promoters
Miniprep cultures from the 2cnd of september was purified with the NucleoSpin Plasmid kit (Macherey-Nagel). DNA was quantify with the Nanodrop 2000.
Glycerol stock were prepared started from the 16 cultures from the 2cnd of september (750 µl of culture + 750 µL of 10% glycerol) and stored at -80°C. Sep 02
