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Team:Paris Bettencourt/Notebook/Odor Library
From 2014.igem.org
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| Line 241: | Line 241: | ||
<div id="June"> | <div id="June"> | ||
<h4>June</h4> | <h4>June</h4> | ||
| - | <h5> | + | <h5>June 26th</h5> |
<h6>Goal</h6> | <h6>Goal</h6> | ||
| - | <p> | + | <p>Design plasmid for limonene synthase</p> |
<h6>Procedure</h6> | <h6>Procedure</h6> | ||
| - | <p> | + | <p>Used MG1665 strain, the RBS with the highest initial rate and the sequence of r-limonene synthase from genBank. Added polyhistidine tail to the construct. Submitted the construct to Jake.</p> |
<h6>Results</h6> | <h6>Results</h6> | ||
| + | <p>Designed plasmid for limonene synthase using the software geneious. Found a biobricks part containing this sequence. For the purpose of saving budget, we would first transform this standard part into cell and later modify it. | ||
| + | |||
| + | </p> | ||
<h5>Date 1</h5> | <h5>Date 1</h5> | ||
<h6>Goal</h6> | <h6>Goal</h6> | ||
Revision as of 14:22, 17 September 2014
Odor Library
Notebook
June
June 26th
Goal
Design plasmid for limonene synthase
Procedure
Used MG1665 strain, the RBS with the highest initial rate and the sequence of r-limonene synthase from genBank. Added polyhistidine tail to the construct. Submitted the construct to Jake.
Results
Designed plasmid for limonene synthase using the software geneious. Found a biobricks part containing this sequence. For the purpose of saving budget, we would first transform this standard part into cell and later modify it.
Date 1
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July
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August
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September
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October
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