Team:Northwestern/Protocols

From 2014.igem.org

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   <li class="active"><a href="#1" role="tab" data-toggle="tab">Digestion</a></li>
   <li class="active"><a href="#1" role="tab" data-toggle="tab">Digestion</a></li>
   <li><a href="#2" role="tab" data-toggle="tab">Ligation and Transformation</a></li>
   <li><a href="#2" role="tab" data-toggle="tab">Ligation and Transformation</a></li>
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   <li><a href="#3" role="tab" data-toggle="tab">Gel extraction</a></li>
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   <li><a href="#3" role="tab" data-toggle="tab">Gel electrophoresis</a></li>
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   <li><a href="#4" role="tab" data-toggle="tab">Making Plates</a></li>
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   <li><a href="#4" role="tab" data-toggle="tab">Gel extraction</a></li>
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  <li><a href="#5" role="tab" data-toggle="tab">Making Plates</a></li>
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<img src="https://static.igem.org/mediawiki/2014/3/37/Flowchart_of_Gel_Extraction.png"/>
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Revision as of 16:40, 16 September 2014

Dropdown menu from bootstrap


Protocols

Note: dephosphorylation with Antarctic phophatase done after digestion. Can use CutSmart for dephosphorylation.

for making competent cells to transform into

we used the following protocol

raybiotech