Revision as of 09:03, 13 September 2014

count down

アメフラシノート

Nakamura Matsumoto

Sample	Volume	EcoR1	Spe1	Buffer	MilliQ	TOTAL
3/24 PCR(dddD)cloning	3.2	1	1	3(H)	21.8	30
pSB1C3	18.5	1	1	3(H)	6.5	30
pSB1C3(control)	6.2	0	0	3(H)	2.8	10

Yasuda Murata

Yasuda

Vector	inserter	10xt4LigaseBuffer	t4Ligase	MilliQ	TOTAL
1.5 μL(8/1 pSB1C3 2047bp 13.3μg/mL 20μg)	6.9 μL(8/1 dddD 2545bp 10.8μg/mL 74.60μg)	2 μL	1 μL	8.6 μL	20 μL

Murata

Name	Sample/(µl)	Competent Cells/(µl)	Total/(µl)	Plate
pSB1C3	3	30	165	SOC(132(µl))

Murata

Tatsui

@@ Line 40: / Line 40: @@
 <table class="note">
 <tr><th>Vector</th><th>inserter</th><th>10xt4LigaseBuffer</th><th>t4Ligase</th><th>MilliQ</th><th>TOTAL</tr>
-<tr><td>(8/1 pSB1C3 2047bp 13.3μg/mL 20μg)1.5 μL </td><td>(8/1 dddD 2545bp 10.8μg/mL 74.60μg)6.9 μL </td><td>2 μL </td><td>1 μL </td><td>8.6 μL </td><td>20 μL </td></tr>
+<tr><td>1.5 μL(8/1 pSB1C3 2047bp 13.3μg/mL 20μg) </td><td>6.9 μL(8/1 dddD 2545bp 10.8μg/mL 74.60μg) </td><td>2 μL </td><td>1 μL </td><td>8.6 μL </td><td>20 μL </td></tr>
+</table>
+<h2>8/1</h2>
+<h3>transformation</h3>
+<span class="author">Murata</span>
+<table class="note">
+<tr><th>Name</th><th>Sample/(&micro;l)</th><th>Competent Cells/(&micro;l)</th><th>Total/(&micro;l)</th><th>Plate</th></tr>
+<tr><td>pSB1C3</td><td>3</td><td>30</td><td>165</td><td>SOC(132(&micro;l))</td></tr>
 </table>