Team:Kyoto/Notebook/DMS Synthesis

From 2014.igem.org

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</table>
</table>
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<h2>8/31</h2>
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<h2>8/1</h2>
<h3>Gel Extraction</h3>
<h3>Gel Extraction</h3>
<span class="author">Yasuda Murata</span>
<span class="author">Yasuda Murata</span>
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<table class="note">
<table class="note">
<tr><th>Vector</th><th>inserter</th><th>10xt4LigaseBuffer</th><th>t4Ligase</th><th>MilliQ</th><th>TOTAL</tr>
<tr><th>Vector</th><th>inserter</th><th>10xt4LigaseBuffer</th><th>t4Ligase</th><th>MilliQ</th><th>TOTAL</tr>
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<tr><td>1.5 μL </td><td>6.9 μL </td><td>2 μL </td><td>1 μL </td><td>8.6 μL </td><td>20 μL </td></tr>
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<tr><td>(8/1 pSB1C3 2047bp 13.3μg/mL 20μg)1.5 μL </td><td>(8/1 dddD 2545bp 10.8μg/mL 74.60μg)6.9 μL </td><td>2 μL </td><td>1 μL </td><td>8.6 μL </td><td>20 μL </td></tr>
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Revision as of 08:57, 13 September 2014

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アメフラシノート

7/31

Restriction Enzyme Digestion

Nakamura Matsumoto
SampleVolumeEcoR1Spe1BufferMilliQTOTAL
3/24 PCR(dddD)cloning3.2113(H)21.830
pSB1C318.5113(H)6.530
pSB1C3(control)6.2003(H)2.810

8/1

Gel Extraction

Yasuda Murata
LaneDNA
11kbp ladder
23/24 PCR(dddD)cloning E,S
33/24 PCR(dddD)cloning E,S
4Blank
5pSB1C3
6pSB1C3
7Blank
81kbp ladder
93/24 PCR(dddD)cloning(control)
10pSB1C3(control)

8/1

Ligation

Yasuda
Vectorinserter10xt4LigaseBuffert4LigaseMilliQTOTAL
(8/1 pSB1C3 2047bp 13.3μg/mL 20μg)1.5 μL (8/1 dddD 2545bp 10.8μg/mL 74.60μg)6.9 μL 2 μL 1 μL 8.6 μL 20 μL

8/3

Electrophoresis for confirmation

Murata
LaneSample
1pSB1C3 dddD 1
2pSB1C3 dddD 2
3pSB1C3 dddD 3
41kb ladder(3μL)
51kb ladder(1μL)

8/3

Gel Extraction

Tatsui
LaneDNA
11kbp ladder
2Blank
3pSB1C3 25μL 2070bp
4Blank
5pSB1C3 5μL
6Blank
78/2 cloning dddD 25μL 2544bp
8Blank
93/24 PCR(dddD)cloning(control)
108/2 cloning dddD 5μL

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