Team:Kyoto/Notebook/DMS Synthesis

From 2014.igem.org

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</table>
</table>
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<h2>8/3</h2>
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<h3>Gel Extraction</h3>
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<span class="author">Tatsui</span>
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<table class="note">
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<tr><th>Lane</th><th>DNA</th>
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<tr><td>1</td><td>1kbp ladder</td></tr>
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<tr><td>2</td><td>Blank</td></tr>
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<tr><td>3</td><td>pSB1C3 25μL 2070bp</td></tr>
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<tr><td>4</td><td>Blank</td></tr>
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<tr><td>5</td><td>pSB1C3 5μL</td></tr>
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<tr><td>6</td><td>Blank</td></tr>
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<tr><td>7</td><td>8/2 cloning dddD 25μL 2544bp</td></tr>
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<tr><td>8</td><td>Blank</td></tr>
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<tr><td>9</td><td>3/24 PCR(dddD)cloning(control)</td></tr>
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<tr><td>10</td><td>8/2 cloning dddD 5μL</td></tr>
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</table>

Revision as of 02:32, 10 September 2014

  1. REDIRECT Template:Kyoto/css
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アメフラシノート

7/31

Restriction Enzyme Digestion

Nakamura Matsumoto
SampleVolumeEcoR1Spe1BuffurMilliQTOTAL
3/24 PCR(dddD)cloning3.2113(H)21.830
pSB1C318.5113(H)6.530
pSB1C3(control)6.2003(H)2.810

8/31

Gel Extraction

Yasuda Murata
LaneDNA
11kbp ladder
23/24 PCR(dddD)cloning E,S
33/24 PCR(dddD)cloning E,S
4Blank
5pSB1C3
6pSB1C3
7Blank
81kbp ladder
93/24 PCR(dddD)cloning(control)
10pSB1C3(control)

8/3

Electrophoresis for confirmation

Murata
LaneSample
1pSB1C3 dddD 1
2pSB1C3 dddD 2
3pSB1C3 dddD 3
41kb ladder(3μL)
51kb ladder(1μL)

8/3

Gel Extraction

Tatsui
LaneDNA
11kbp ladder
2Blank
3pSB1C3 25μL 2070bp
4Blank
5pSB1C3 5μL
6Blank
78/2 cloning dddD 25μL 2544bp
8Blank
93/24 PCR(dddD)cloning(control)
108/2 cloning dddD 5μL

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