Team:Kyoto/Notebook/DMS Synthesis
From 2014.igem.org
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<tr><td>9</td><td>3/24 PCR(dddD)cloning(control)</td></tr> | <tr><td>9</td><td>3/24 PCR(dddD)cloning(control)</td></tr> | ||
<tr><td>10</td><td>pSB1C3(control)</td></tr> | <tr><td>10</td><td>pSB1C3(control)</td></tr> | ||
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Revision as of 02:19, 10 September 2014
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アメフラシノート
7/31
Restriction Enzyme Digestion
Sample | Volume | EcoR1 | Spe1 | Buffur | MilliQ | TOTAL |
---|---|---|---|---|---|---|
3/24 PCR(dddD)cloning | 3.2 | 1 | 1 | 3(H) | 21.8 | 30 |
pSB1C3 | 18.5 | 1 | 1 | 3(H) | 6.5 | 30 |
pSB1C3(control) | 6.2 | 0 | 0 | 3(H) | 2.8 | 10 |
8/31
Gel Extraction
Lane | DNA |
---|---|
1 | 1kbp ladder |
2 | 3/24 PCR(dddD)cloning E,S |
3 | 3/24 PCR(dddD)cloning E,S |
4 | Blank |
5 | pSB1C3 |
6 | pSB1C3 |
7 | Blank |
8 | 1kbp ladder |
9 | 3/24 PCR(dddD)cloning(control) |
10 | pSB1C3(control) |