Team:Sheffield/WLjournal

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<h5>Monday 23<sup>rd</sup> June</h5>
<h5>Monday 23<sup>rd</sup> June</h5>
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Make up 100ml of <a href="#" onClick="MyWindow=window.open('http://imgur.com','MyWindow',
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Make up 100ml of <a href="#" onClick="MyWindow=window.open('https://2014.igem.org/Team:Sheffield/Prot1','MyWindow',
'toolbar=no,location=no,directories=no,status=no, menubar=no,scrollbars=no,resizable=no,width=600,height=300');  
'toolbar=no,location=no,directories=no,status=no, menubar=no,scrollbars=no,resizable=no,width=600,height=300');  
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return false;" style="color:#DAA520;text-decoration:none">LB Broth</a> and 100ml <a href="#" onClick="MyWindow=window.open('http://imgur.com','MyWindow',
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return false;" style="color:#DAA520;text-decoration:none">LB Broth</a> and 100ml <a href="#" onClick="MyWindow=window.open('https://2014.igem.org/Team:Sheffield/Prot2','MyWindow',
'toolbar=no,location=no,directories=no,status=no, menubar=no,scrollbars=no,resizable=no,width=600,height=300');  
'toolbar=no,location=no,directories=no,status=no, menubar=no,scrollbars=no,resizable=no,width=600,height=300');  
return false;" style="color:#DAA520;text-decoration:none">LB Agar</a>.
return false;" style="color:#DAA520;text-decoration:none">LB Agar</a>.
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<br><br>Make an <a href="#" onClick="MyWindow=window.open('http://imgur.com','MyWindow',
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<br><br>Make an <a href="#" onClick="MyWindow=window.open('https://2014.igem.org/Team:Sheffield/Prot','MyWindow',
'toolbar=no,location=no,directories=no,status=no, menubar=no,scrollbars=no,resizable=no,width=600,height=300');  
'toolbar=no,location=no,directories=no,status=no, menubar=no,scrollbars=no,resizable=no,width=600,height=300');  
return false;" style="color:#DAA520;text-decoration:none">overnight</a> starter culture of BBa_K258008 to test sterile technique.  
return false;" style="color:#DAA520;text-decoration:none">overnight</a> starter culture of BBa_K258008 to test sterile technique.  

Revision as of 20:06, 9 September 2014

Week 1

Monday 23rd June
Make up 100ml of LB Broth and 100ml LB Agar.

Make an overnight starter culture of BBa_K258008 to test sterile technique.
Tuesday 24th June
Generate Chemically competent E.Coli.

Make up 8 flasks of 100ml starter culture.
4 flasks were checked each time to take the OD readings.
OD readings to show when the cells are in exponential growth phase and so ready to be made competent.

TABLE
A B C D 1.25h 0.077 0.078 0.082 0.096 3.25h 0.567 0.54 0.674 0.543 0.714 0.837 0.809 0.814

Aliquot into 24 falcon tubes. The cells will need to be spun down in 2 rounds.
72 eppendorf tubes will be produced, labelled ch. comp, dh5alpha.
Store at -80°C.

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