Team:Sheffield/WLjournal

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Revision as of 19:55, 9 September 2014

Week 1

Monday 23rd June
Make up 100ml of LB Broth and 100ml LB Agar.

Make an overnight starter culture of BBa_K258008 to test sterile technique.
Tuesday 24th June
Generate Chemically competent E.Coli.

Make up 8 flasks of 100ml starter culture.
4 flasks were checked each time to take the OD readings.
OD readings to show when the cells are in exponential growth phase and so ready to be made competent.

TABLE
A B C D 1.25h 0.077 0.078 0.082 0.096 3.25h 0.567 0.54 0.674 0.543 0.714 0.837 0.809 0.814

Aliquot into 24 falcon tubes. The cells will need to be spun down in 2 rounds.
72 eppendorf tubes will be produced, labelled ch. comp, dh5alpha.
Store at -80°C.

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