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Team:Evry/Notebook/Sensing/07-29-2014
From 2014.igem.org
(Difference between revisions)
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<img src="https://static.igem.org/mediawiki/2014/9/96/Pouet2.jpg" alt="pouet" /> <br> | <img src="https://static.igem.org/mediawiki/2014/9/96/Pouet2.jpg" alt="pouet" /> <br> | ||
| - | 1.Serial dilutions of compound was made from 10(-2) mol/L to 10(-8) mol/L <br> | + | 1. Serial dilutions of compound was made from 10(-2) mol/L to 10(-8) mol/L <br> |
<li><img src="https://static.igem.org/mediawiki/2014/6/6d/Dilutionlead.jpg" alt="text to print if image not found" /> <br> | <li><img src="https://static.igem.org/mediawiki/2014/6/6d/Dilutionlead.jpg" alt="text to print if image not found" /> <br> | ||
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<br> | <br> | ||
| - | 2.300µL of E.coli BL21 was added in each eppendorf tube.<br> | + | 2. 300µL of E.coli BL21 was added in each eppendorf tube.<br> |
We had 2 control tubes: | We had 2 control tubes: | ||
A negative control: 2,7mL of MB medium + 300µL of compound | A negative control: 2,7mL of MB medium + 300µL of compound | ||
A positive control: 2,7mL of MB medium + 300µL of E.coli BL21 | A positive control: 2,7mL of MB medium + 300µL of E.coli BL21 | ||
| - | + | <br> | |
| - | 3.Incubation of tubes at 30°C overnight, 200rpm | + | <br> |
| + | 3. Incubation of tubes at 30°C overnight, 200rpm | ||
</p> | </p> | ||
Revision as of 09:56, 8 September 2014
Survivability in molecules which are going to be sensed:
Ranges of concentrations of molecules that we want to sense were made as described in following tables:
1. Serial dilutions of compound was made from 10(-2) mol/L to 10(-8) mol/L
2. 300µL of E.coli BL21 was added in each eppendorf tube.
We had 2 control tubes: A negative control: 2,7mL of MB medium + 300µL of compound A positive control: 2,7mL of MB medium + 300µL of E.coli BL21
3. Incubation of tubes at 30°C overnight, 200rpm Jul 29
