Team:Evry/Notebook/Sensing/PCBs/08-14-2014
From 2014.igem.org
(Difference between revisions)
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- | <u>Survival test on E.coli BL21:</u> | + | <b><u>Survival test on E.coli BL21:</u></b> |
+ | <br/> | ||
<br/>This test allowed to know if E.coli can survive at different concentrations of 2 hydroxy-3',4'dichlorobiphenyl | <br/>This test allowed to know if E.coli can survive at different concentrations of 2 hydroxy-3',4'dichlorobiphenyl | ||
<ol> | <ol> | ||
- | <li> | + | <li>For that, BL21 culture was removed from the -80°C and culturing in 4 mL of LB |
- | <li> | + | <li>E.coli was incubated overnight at 37°C, 200rpm |
</ol> | </ol> | ||
Latest revision as of 10:45, 6 September 2014
Survival test on E.coli BL21:
This test allowed to know if E.coli can survive at different concentrations of 2 hydroxy-3',4'dichlorobiphenyl
- For that, BL21 culture was removed from the -80°C and culturing in 4 mL of LB
- E.coli was incubated overnight at 37°C, 200rpm