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Team:Evry/Notebook/Sensing/PCBs/07-28-2014
From 2014.igem.org
(Difference between revisions)
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<p align="justify"> | <p align="justify"> | ||
| - | <u>Sensor construction bphR1/ | + | <b><u>Sensor construction bphR1/PbphR2:</u></b> |
<ol> | <ol> | ||
| - | <li>Plate 20µL on LB-agar-Cam (25 mL LB-Agar + 25µL | + | <li>Plate 20µL from the culture of <a href="https://2014.igem.org/Team:Evry/Notebook/Sensing/PCBs/07-27-2014">27th july</a> on LB-agar-Cam (25 mL LB-Agar + 25µL Chloramphenicol) |
<li>Incubation of plate at 37°C overnight | <li>Incubation of plate at 37°C overnight | ||
| - | <li>A glycerol stock was made with 750ml of culture and 750ml of glycerol 50% | + | <li>A glycerol stock was made with 750ml of culture and 750ml of glycerol 50% and stored at -80°C |
</ol> | </ol> | ||
Revision as of 10:29, 6 September 2014
Sensor construction bphR1/PbphR2:
- Plate 20µL from the culture of 27th july on LB-agar-Cam (25 mL LB-Agar + 25µL Chloramphenicol)
- Incubation of plate at 37°C overnight
- A glycerol stock was made with 750ml of culture and 750ml of glycerol 50% and stored at -80°C
