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Team:UCL/protocols
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<p><b>Materials</b><br/> | <p><b>Materials</b><br/> | ||
| - | + | LB, Falcon Tubes, Ice, Chilled centrifuge, CaCL, Microtubes (300ul/tube)<br/><br/> | |
| - | LB | + | |
| - | Falcon Tubes | + | |
| - | Ice | + | |
| - | Chilled centrifuge | + | |
| - | CaCL | + | |
| - | Microtubes (300ul/tube)<br/> | + | |
<b>Procedure</b><br/> | <b>Procedure</b><br/> | ||
Inoculate a single colony into 5ml Lb in 50ml falcon tube. Grown O/N @ 37oC<br/> | Inoculate a single colony into 5ml Lb in 50ml falcon tube. Grown O/N @ 37oC<br/> | ||
Use 1ml to inoculate 100ml of LB in 250ml bottle the next morning.<br/> | Use 1ml to inoculate 100ml of LB in 250ml bottle the next morning.<br/> | ||
| - | Shake @ 37oC for 1.5-3 hours.<br/> | + | Shake @ 37oC for 1.5-3 hours.<br/><br/> |
Or<br/> | Or<br/> | ||
Inoculate a single colony into 25ml LB in a 250ml bottle in the morning<br/> | Inoculate a single colony into 25ml LB in a 250ml bottle in the morning<br/> | ||
| - | Shake @ 37oC for 4-6 hours.<br/> | + | Shake @ 37oC for 4-6 hours.<br/><br/> |
| - | Then…<br/> | + | Then…<br/><br/> |
Put the cells on ice for 10mins (keep cold from now on).<br/> | Put the cells on ice for 10mins (keep cold from now on).<br/> | ||
Revision as of 14:32, 5 September 2014
