Team:ETH Zurich/labblog/20140825meet

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* We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.
* We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.
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Revision as of 10:05, 5 September 2014

Week 14

Monday, August 25th

  • We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.


Diffusion experiment with liquid. Check the corresponding article for more details.
Diffusion experiment with beads. Check the corresponding article for more details.


  • We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length.
Comsol simulation of diffusion experiments for 1mM and for 1 μM AHL initial concentration in sender wells. It explains why with 1mM all wells start glowing at the same time.
  • We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves.
Fitting of quorum sensing dynamic parameters. Check the corresponding article for more details.

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