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Team:Paris Saclay/Notebook/September/1
From 2014.igem.org
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We had a problem to clone LS in pGMETeasy and pPSI. So from the dish of streaking from [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/26#Streaking_of_colonies_transformed_by_BBa_K762100.2BpGEMTeasy_.28LS.29 26 August]We do PCR of all the clones: | We had a problem to clone LS in pGMETeasy and pPSI. So from the dish of streaking from [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/26#Streaking_of_colonies_transformed_by_BBa_K762100.2BpGEMTeasy_.28LS.29 26 August]We do PCR of all the clones: | ||
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| - | PPS5 | + | ====PPS5==== |
We check if we have the right insert in our plasmid pPS5 : CAD (cinnalyl alcool deshydrogenase) | We check if we have the right insert in our plasmid pPS5 : CAD (cinnalyl alcool deshydrogenase) | ||
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| - | PPS3 and PPS4 | + | ====PPS3 and PPS4==== |
We need to work on this plasmid tomorrow so we do some culture from the stock | We need to work on this plasmid tomorrow so we do some culture from the stock | ||
Revision as of 15:42, 4 September 2014
Contents |
Lab Work
B-Construction of the fusion protein
'by Hoang Vu'
We pricked out white colonies from Xgal-IPTG petri dish and blue colonies found in non Xgal-IPTG petri dish in a petri dish that contain Ampicillin to check if the blue ones are not came from a carrying of Xgal-IPTG during the precedent prick out.
D-Lemon scent
by melanie
Limone synthase
We had a problem to clone LS in pGMETeasy and pPSI. So from the dish of streaking from 26 AugustWe do PCR of all the clones:
We use the Protocol - PCR for bacterial culture and we use the same PCR condition than on the August 8 but with the appropriate Primer and Biobrick.
PPS5
We check if we have the right insert in our plasmid pPS5 : CAD (cinnalyl alcool deshydrogenase) So we do a PCR in the same condition than August 20
PPS3 and PPS4
We need to work on this plasmid tomorrow so we do some culture from the stock
