Team:Evry/Notebook/CellCharacterization/Antibiotic test/08-17-2014
From 2014.igem.org
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<b><u>Tests of antibiotics' stocks - Results</u></b> <br> | <b><u>Tests of antibiotics' stocks - Results</u></b> <br> | ||
<table border="1"> | <table border="1"> | ||
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<td> <b>Plates </b> </td> | <td> <b>Plates </b> </td> | ||
<td>LB Agar only </td> | <td>LB Agar only </td> | ||
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<b><u>Survivability tests - Results</u></b> <br> | <b><u>Survivability tests - Results</u></b> <br> | ||
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<table border="1"> | <table border="1"> | ||
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<td> <b>liquid cultures/Plates </b> </td> | <td> <b>liquid cultures/Plates </b> </td> | ||
<td> <b>MB </b> </td> | <td> <b>MB </b> </td> |
Revision as of 15:19, 4 September 2014
Tests of antibiotics' stocks - Results
Plates | LB Agar only | LB+Cam | LB+Kan | LB+Amp | LB+Erm | LB+Tet |
E.coli | X | 0 | X | 0 | X | 0 |
There was a problem with the stock of Kanamycine so we made a new one at 25mg/mL. For the erythrompycine, we learn that E. Coli is not really sensitive to this antibiotic and that it's better to use it with a dilution at 1:100.
Survivability tests - Results
liquid cultures/Plates | MB | M9 |
MB | ++++ | ++ |
M9 | ++ | + |
Any liquid culture can be plated and will grow up. Of course, it works better on MB medium, wich is a rich medium than on M9. Aug 17