Overview

Impact of pest

In agriculture, insect problem has existed for a long time, and has been difficult to be solved, all of the agricultural products all over the world every year cause serious harm and damage caused by these insects bulky and difficult to estimate.

Losses caused by pests on crops included direct damage, drug costs and the labor costs, The most difficult to estimate the loss of pesticides on human health hazards caused by consuming a lot more medical resources.

We can see this a credible chart, insect damage recorded in Brazil region, pests generally reduce crop yields by 10 percent, which is each crop have reduced the value of hundreds of millions. Our goal is committed to reducing agricultural losses, safeguard human health, and to maintain ecological balance.

Pesticide hazards

Because of the hazard of insects, human beings to make a lot of ideas to kill these harmful insects. In 15th century,people used heavy metals to kill harmful insects,like Arsenic,Mercury and Plumbum, which cause a catastrophe to the environment. Pesticide become more powerful along with the technology. In 20th century,the agriculture develops rapidly just because of the evolution of pesticide. But the pesticide is not only harmful to the insects but also harmful to the human beings. People found this problem after several decades. The toxin of the pesticide will be kept in creatures by the food chain,and finally go into human's body. However,it's not too late to improve this situation. We can create an evolution of agriculture by a new method,and it is what we do!

Solution to both questions

In order to solve the pest need to use pesticides, but the use of pesticides will destroy the Earth and cause harm to humans. Thus there has been conflicting issues, and has been difficult to be solved. In order to solve the problems we mentioned, we came up with a practical, inexpensive way to solve. Figure,1 is a diagram of our device, after which we will mention how this device to bring better results for our plan and improve his efficiency.

Figure,1 Our Device

PBAN

Introduction

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Design of Red promoter

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Reference

Biobrick Design

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We searched the DNA sequence of the PBANs from moths on NCBI, then contrasted to the amino sequences from papers so that we can selected the DNA fragments which directly correspond to gland-stimulating function, By ligating Ribosome binding site(B0034) and PBAN DNA sequence, we were able to make E.coli directly produce these PBANs instead of the original complex process of PBAN biosynthesis in insects. We had gotten nine kinds of PBANs,each of which is from one kind of moth ,after we constructed the PBAN biobricks, the B0034+BFP+J61048 biobrick was ligated behind the PBAN biobrick in order to model each PBAN biobrick respectively in the future and make observing the production of PBANs more convenient for us.

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Cover image credit: DVQ