Team:Evry/Notebook/Sensing/Phenol/08-15-2014
From 2014.igem.org
(Difference between revisions)
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BBa_k1031222 (P0-RBS B0032-GFP)<br> | BBa_k1031222 (P0-RBS B0032-GFP)<br> | ||
Liquid : 3ml of Luria Broth 1x + 34µg/L Chloramphenicol in a 15ml falcon at 37°C shaking <br> | Liquid : 3ml of Luria Broth 1x + 34µg/L Chloramphenicol in a 15ml falcon at 37°C shaking <br> | ||
- | On plate : LB 1x | + | On plate : 25ml LB 1x + 25ml |
+ | Agarose 1x + 34µg/L Chloramphenicol <br> | ||
Observation: DmpR bacteria have difficulties to grow compared with GFP bacteria <br> | Observation: DmpR bacteria have difficulties to grow compared with GFP bacteria <br> | ||
- | + | <img src="https://static.igem.org/mediawiki/2014/0/07/Evry_2014_B0031_b0032.jpg" <br> | |
+ | Left : BBa_K1031221 (P0-RBS B0031-GFP)<br> | ||
+ | Right : BBa_k1031222 (P0-RBS B0032-GFP)<br> | ||
</p> | </p> | ||
Revision as of 13:36, 2 September 2014
TITLE HERE !!!!!
Cell culture of E.coli received from registry
BBa_K1031211 (Pr-DmpR )
BBa_K1031221 (P0-RBS B0031-GFP)
BBa_k1031222 (P0-RBS B0032-GFP)
Liquid : 3ml of Luria Broth 1x + 34µg/L Chloramphenicol in a 15ml falcon at 37°C shaking
On plate : 25ml LB 1x + 25ml
Agarose 1x + 34µg/L Chloramphenicol
Observation: DmpR bacteria have difficulties to grow compared with GFP bacteria
Left : BBa_K1031221 (P0-RBS B0031-GFP)
Right : BBa_k1031222 (P0-RBS B0032-GFP)