Team:NCTU Formosa/Safty

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(Researcher Safety)
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===Researcher Safety===
 
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===Light-regulated System===
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1.Everyone must wear lab coat, trousers, gloves, surgery masks and shoes when carrying out experiments.
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2.Before the commencement of our project,our team instructors and advisers have guided us to go through every expiriment to make sure everybody is well trained and fit for this project.
 
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3.Everybody has to sterilize his/her hands and all the equipment platforms with alcohol solution(70%) before and after each expiriment.
 
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4.Emergency equipments are well prepared and all the researchers understand how to use these equipments and their exact position.
 
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5.It is not allowed that researcher carries experiment alone.There must be at least two members to conduct the experiment.
 
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6.Food and beverages are not allowed to appear in the lab.
 
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====Red Promoter====
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===Public safety===
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===Temperature-regulated System===
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1.All collected lab waste are sterilized, packaged and executed.<br>
 
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2.E.coli used in our lab is less competitive than wild type, because they are unable to form biofilms and to thrive in the intestine.<br>
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====37 °C RBS====
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3.Liquid waste that contains E.coli is disinfected by adding peroxides and cleaners before it is disposed.<br>
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===Instruments in our lab===
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===Small RNA-regulated System===
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====rRBS efficiency====
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1.incubator<p>
 
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The orbital shaker incubator is widely used in microbiology labs, cell biology labs, etc. It provides a stationary temperature to cultivate bacteria and cells. Some incubator also have functions to maintain the humility level.
 
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In our lab, the incubator is maintaining the temperature at 37 degrees Celsius, which is the optical temperature of E.coli growing.
 
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In this year, our team was authorized to work with an non-pathogenic bacterial strain, the Escherichia coli DH5. The strain ais regularly employed in research, industry and study. Biosafety Level 1 standard regulations were strictly followed while using these strains.</p>
 
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====Effect on ''E. Coli'' Growth====
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====Expected sRNA regulation efficiency====
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     <p>Cover image credit: <a href="http://www.dvq.co.nz/" target="_blank">DVQ</a></p>
     <p>Cover image credit: <a href="http://www.dvq.co.nz/" target="_blank">DVQ</a></p>
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Revision as of 01:57, 2 September 2014

Results

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Contents

Light-regulated System

Red Promoter

Temperature-regulated System

37 °C RBS

Small RNA-regulated System

rRBS efficiency

Effect on E. Coli Growth

Expected sRNA regulation efficiency