Team:ETH Zurich/labblog/20140825meet
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* We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. | * We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. | ||
- | [[File:ETH Zurich Diffusion2.JPG|center|350px|thumb|Diffusion experiment with agar. Check <html> <a class="all" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html> for more details.]] | + | [[File:ETH Zurich Diffusion2.JPG|center|350px|thumb|Diffusion experiment with agar. Check <html> <a class="all" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html> for more details.]] |
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+ | [[File:ETH Zurich Diffusion4.JPG|center|350px|thumb|Diffusion experiment with beads. Check <html> <a class="all" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html> for more details.]] | ||
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Revision as of 14:08, 1 September 2014
Week 14
Monday, August 25th
- We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.
- We have a Comsol simulation for this experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length.
- We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves.