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Team:Evry/Notebook/CellCharacterization/Transformation/08-23-2014
From 2014.igem.org
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| - | <img src="" alt="pBBR1MCS"/> | + | <img src="https://static.igem.org/mediawiki/2014/e/e1/PBBR1MCS.JPG" alt="pBBR1MCS"/> |
| - | <img src="" alt="pRhokH1-2"/> | + | <img src="https://static.igem.org/mediawiki/2014/2/27/PRhokHi-2.JPG" alt="pRhokH1-2"/> |
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Revision as of 17:26, 28 August 2014
Amplification of plasmids pBBR1MCS and pRhokHI-2
Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology)
Bowth were transfered into Bl21 electro-competent cells by electroporation (LIEN PROTO)(two replicate).
After a liquid culture of three hours, cells were plated on MB 1X + Cam (1:1000) and let in incubator overnight. Aug 23
