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Team:Evry/Interlab Study/08-22-2014
From 2014.igem.org
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<br/> Transformation plate observation: | <br/> Transformation plate observation: | ||
| - | <br/>- BBa_J23101: | + | <br/>- BBa_J23101: 11 isolated colonies |
<br/>- BBa_K823012: 100-150 isolated colonies | <br/>- BBa_K823012: 100-150 isolated colonies | ||
<br/> | <br/> | ||
| - | <br/> A PCR was performed on 8 colonies for BBa_K823012 following the protocol Table 3 and 4. | + | <br/> A PCR was performed on 8 colonies for BBa_K823012 and for BBa_J23101 following the protocol Table 3 and 4. |
<br/> To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X. | <br/> To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X. | ||
Revision as of 17:26, 28 August 2014
Contructions PSB1C3 with I20260, J23101-E1010 and K823012-E1010
Transformation plate observation:
- BBa_J23101: 11 isolated colonies
- BBa_K823012: 100-150 isolated colonies
A PCR was performed on 8 colonies for BBa_K823012 and for BBa_J23101 following the protocol Table 3 and 4.
To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X.
We expected to obtain a band between 300 and 400 bp. Aug 22
