Team:Evry/Notebook/CellCharacterization/Transformation/08-23-2014
From 2014.igem.org
(Difference between revisions)
Line 16: | Line 16: | ||
</div> | </div> | ||
<br> | <br> | ||
- | Bowth were transfered into Bl21 electro-competent cells by electroporation (LIEN PROTO)(two replicate). After a liquid culture of three hours, cells were plated on MB 1X + Cam (1:1000) and let in incubator overnight. | + | Bowth were transfered into Bl21 electro-competent cells by electroporation <b>(LIEN PROTO)</b>(two replicate). <br> After a liquid culture of three hours, cells were plated on MB 1X + Cam (1:1000) and let in incubator overnight. |
Revision as of 16:31, 28 August 2014
Amplification of plasmids pBBR1MCS and pRhokHI-2
Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology)
Bowth were transfered into Bl21 electro-competent cells by electroporation (LIEN PROTO)(two replicate).
After a liquid culture of three hours, cells were plated on MB 1X + Cam (1:1000) and let in incubator overnight. Aug 23