Team:ETH Zurich/labblog/20140825meet
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* We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. | * We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. | ||
[[File:ETH Zurich Diffusion4.JPG|400px]] [[File:ETH Zurich Diffusion3.JPG|400px]] [[File:ETH Zurich Diffusion2.JPG|400px]] | [[File:ETH Zurich Diffusion4.JPG|400px]] [[File:ETH Zurich Diffusion3.JPG|400px]] [[File:ETH Zurich Diffusion2.JPG|400px]] | ||
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+ | *We have a Comsol simulation for this experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length. | ||
+ | [[File:ETH Zurich Comsol3.PNG|900px]] | ||
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Revision as of 12:38, 28 August 2014
Week 14
Monday, August 25th
- We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.
- We have a Comsol simulation for this experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length.