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Team:Evry/Interlab Study/08-22-2014
From 2014.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
<br/> Transformation plate observation: | <br/> Transformation plate observation: | ||
<br/>- BBa_J23101: nothing grown | <br/>- BBa_J23101: nothing grown | ||
| - | <br/>- | + | <br/>- BBa_K823012: 100-150 isolated colonies |
<br/> | <br/> | ||
| - | <br/> A PCR was performed on 8 colonies for | + | <br/> A PCR was performed on 8 colonies for BBa_K823012 following the protocol Table 3 and 4. |
<br/> To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X. | <br/> To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X. | ||
Revision as of 15:56, 28 August 2014
Transformation plate observation:
- BBa_J23101: nothing grown
- BBa_K823012: 100-150 isolated colonies
A PCR was performed on 8 colonies for BBa_K823012 following the protocol Table 3 and 4.
To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X.
We expected to obtain a band between 300 and 400 bp. Aug 22
