Team:Evry/Notebook/Sensing/PCBs/08-22-2014
From 2014.igem.org
(Difference between revisions)
(Created page with "<html> <div class="cd-timeline-block"> </html> {{:Team:Evry/Notebook/Biology/TopImage}} <html> <div class="cd-timeline-content"> <p align="justify"> Transformation pl...") |
|||
Line 2: | Line 2: | ||
<div class="cd-timeline-block"> | <div class="cd-timeline-block"> | ||
</html> | </html> | ||
- | {{:Team:Evry/Notebook/ | + | {{:Team:Evry/Notebook/Sensing/PCBs/TopImage}} |
<html> | <html> | ||
<div class="cd-timeline-content"> | <div class="cd-timeline-content"> |
Revision as of 13:37, 25 August 2014
Transformation plate observation:
- BBa_E1010: 50-60 colonies
- BBa_J23114: 150-200 colonies
- BBa_B0015: 40-50 colonies
- PSB1C3G3: > 1000 red colonies
A PCR was performed on 8 colonies for BBa_J23115 (K823012) following the protocol Table 3 and 4.
Samples are loaded on a 1% agarose gel, 10 µL of sample + 2 µl of loading dye 6X per well. Gel ran 45 minutes at 100 mV.