Team:UT-Dallas/Notebook/8-22
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- | <LI> B3 reporter vectors on Chlora: Glycerol | + | <LI> B3 reporter vectors on Chlora: Inoculate |
- | <LI> Inoculate | + | <LI> B4 reporters-chlora: Glycerol stock + Miniprep + digest + gel extract + purify + ligate + transform onto carb back bone. |
- | + | <LI> Inoculate tcpA(3)-chlora reporter. | |
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</UL> | </UL> | ||
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Revision as of 22:15, 22 August 2014
Friday, August 22, 2014
Today is also long day. (We almost repeated everything we did yesterday: inoculation, miniprep, ligation, and transformation. Also, sequencing results are here. Second batch (B2):
Third batch (B3): Yesterday, we transformed reporter-Carb; today, we inoculate them. The colonies are not very big, we have about 3 colonies on the negative; however, the other plates are very good: they has at least twice-fold the colonies on the negative. Forth batch (B4): We inoculated yesterday. We are minipreping, digesting, ligating onto Carb back bone, and transforming them tcpQ is being re-digested and re-tested on 2% gel to make sure of our clone. We tested it on 1% gel but it created a smear and was dubious. Ran out of PstI-HF!!! Need to order!!! Tomorrow: For B4 reporters and B3 gRNA, inoculation. B3 reporters: miniprep. Sunday, we will miniprep. Next week, on Monday, we should have everything ready for test digest and then finally send the last batch of sequencing! |
Today's tasks: | |
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Terrible, terrible gel! We punctured the gel! The lane bled over each other. We had to make another gel to purify. (In order, i5.47.1-2-3-4-X-5-6-7-8-9-10-11-12) Updating the wiki notebook from the slowest computer in the building because currently we can't log into any other computer. Rishika is gel-ing the ctx-s. |