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Team:UNIK Copenhagen/Logbook
From 2014.igem.org
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<p><b>17th of July - amplification of pETDuet-1 (Q)</b><br> | <p><b>17th of July - amplification of pETDuet-1 (Q)</b><br> | ||
We transformed Ecloni with pETDuet-1 vector and plated the bacteria. They were afterwards stored in the fridge.<br><br> | We transformed Ecloni with pETDuet-1 vector and plated the bacteria. They were afterwards stored in the fridge.<br><br> | ||
| - | <b>23rd of July (Q)</b><br>We made | + | <b>23rd of July (Q)</b><br>We made three liquid cultures each from a single colony on the plate.<br><br> |
<b>24th of July(Q)</b><br>Miniprep of the pETDuet-1 vector. The minipreps was nanodroped. The measurements were:<br></p> | <b>24th of July(Q)</b><br>Miniprep of the pETDuet-1 vector. The minipreps was nanodroped. The measurements were:<br></p> | ||
| - | <table> | + | <table><tr> |
| + | <td>Colony:</td> | ||
| + | <td>ng/iul</td> | ||
| + | <td>260/280</td> | ||
| + | <td>230/260</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>1</td> | ||
| + | <td>30.3</td> | ||
| + | <td>??</td> | ||
| + | <td>??</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>2</td> | ||
| + | <td>??</td> | ||
| + | <td>??</td> | ||
| + | <td>???</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>3</td> | ||
| + | <td>??</td> | ||
| + | <td>??</td> | ||
| + | <td>??</td> | ||
| + | </tr></table> | ||
</div> | </div> | ||
<div class="right_page"> | <div class="right_page"> | ||
Revision as of 12:26, 22 August 2014
17th of July - amplification of pETDuet-1 (Q)
We transformed Ecloni with pETDuet-1 vector and plated the bacteria. They were afterwards stored in the fridge.
23rd of July (Q)
We made three liquid cultures each from a single colony on the plate.
24th of July(Q)
Miniprep of the pETDuet-1 vector. The minipreps was nanodroped. The measurements were:
| Colony: | ng/iul | 260/280 | 230/260 |
| 1 | 30.3 | ?? | ?? |
| 2 | ?? | ?? | ??? |
| 3 | ?? | ?? | ?? |
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