"
Team:Paris Bettencourt/Notebook/Eliminate Smell
From 2014.igem.org
(Difference between revisions)
| Line 278: | Line 278: | ||
<p>Using Geneious, we first created the agaA construct: | <p>Using Geneious, we first created the agaA construct: | ||
| - | <ul>Promoter BBa_J23108 from the Anderson's promoter collection</ | + | <ul><li>Promoter BBa_J23108 from the Anderson's promoter collection</li> |
| - | < | + | <li>RBS from the RBS Calculator of Salis lab specific for E coli</li> |
| - | < | + | <li>BioBrick Prefix + Scar from the iGEM Parts webpage</li> |
| - | < | + | <li>agaA sequence codon optimized for E coli 12 (IDT online tool). We checked with Generious that there were no restriction sites for EcoRI, SpeI, ZbaI and PstI.</li> |
| - | < | + | <li>Histidine tag: 6 Histidines in C-terminal were added</li> |
| - | < | + | <li>Stop codon</li> |
| - | < | + | <li>BioBrick scar + BioBrick suffix from the iGEM Parts webpage</li></ul> |
</p> | </p> | ||
</br> | </br> | ||
Revision as of 14:07, 19 August 2014
Paris Bettencourt 2014
| Home | Team | Official Team Profile | Project | Parts | Modeling | Notebook | Safety | Attributions |
Eliminate Smell
Notebook
June
June 23rd
Goal: Design plasmids that express agaA construct
Procedure:
Using Geneious, we first created the agaA construct:
- Promoter BBa_J23108 from the Anderson's promoter collection
- RBS from the RBS Calculator of Salis lab specific for E coli
- BioBrick Prefix + Scar from the iGEM Parts webpage
- agaA sequence codon optimized for E coli 12 (IDT online tool). We checked with Generious that there were no restriction sites for EcoRI, SpeI, ZbaI and PstI.
- Histidine tag: 6 Histidines in C-terminal were added
- Stop codon
- BioBrick scar + BioBrick suffix from the iGEM Parts webpage
We will set the parameters in the machine and run the reaction overnight.
Text
Date 2
Text
Text
July
Date 1
Text
Text
Date 2
Text
Text
August
Date 1
Text
Text
Date 2
Text
Text
September
Date 1
Text
Text
Date 2
Text
Text
October
Date 1
Text
Text
Date 2
Text
Text
