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Team:Evry/Notebook/Sensing/PCBs/week5
From 2014.igem.org
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<br/> Culturing bphR1 promoter (BBa_K1155001), received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam) | <br/> Culturing bphR1 promoter (BBa_K1155001), received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam) | ||
<br/> Incubation at 37°C overnight | <br/> Incubation at 37°C overnight | ||
| - | <br/> Plate of 1 colony on LB-agar-Cam (25 | + | <br/> Plate of 1 colony on LB-agar-Cam (25 mL LB-Agar + 25µL Cam) |
<br/> Incubation at 37°C overnight and glycerol stock | <br/> Incubation at 37°C overnight and glycerol stock | ||
Revision as of 16:03, 18 August 2014
Week 5
Sensor construction bphR1/bphR2
bphR1 promoter:
Culturing bphR1 promoter (BBa_K1155001), received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam)
Incubation at 37°C overnight
Plate of 1 colony on LB-agar-Cam (25 mL LB-Agar + 25µL Cam)
Incubation at 37°C overnight and glycerol stock
Sensor construction bphR1/bphR2
bphR1 promoter:Culturing bphR1 promoter (BBa_K1155001), received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam)
Incubation at 37°C overnight
Plate of 1 colony on LB-agar-Cam (25 mL LB-Agar + 25µL Cam)
Incubation at 37°C overnight and glycerol stock
