Team:HIT-Harbin/Judging

From 2014.igem.org

(Difference between revisions)
Line 91: Line 91:
</div>
</div>
<div id="bottomNav"><a href="#top">Back&nbsp;To&nbsp;Top</a></div>
<div id="bottomNav"><a href="#top">Back&nbsp;To&nbsp;Top</a></div>
-
<div id="rightCentent">
 
-
<!--Rightnavigation -->
 
-
  <div id="rnav">
 
-
    <!--project -->
 
-
    <div id="rproject" class="rnav1"  onmouseover="sideMenu(1)">                                <a href="https://2014.igem.org/Team:HIT-Harbin/Project" name="top">Project</a>
 
-
      <ul name="rnav">
 
-
        <li>Abstract</li>
 
-
        <li>Background</li>
 
-
        <li>Design</li>
 
-
        <li>Modeling</li>
 
-
        <li>Results</li>
 
-
        <li class="rfoot">Future Work</li>
 
-
      </ul>
 
-
    </div>
 
-
    <!--Judging-->
 
-
    <div id="rjudging" class="rnav1"  onmouseover="sideMenu(2)" ><a href="https://2014.igem.org/Team:HIT-Harbin/Judging">Judging</a>
 
-
      <ul name="rnav">
 
-
        <li>Biobrick</li>
 
-
        <li class="rfoot">Achievement</li>
 
-
    </div>
 
-
    <!--Wetlab-->
 
-
    <div id="rwetlab" class="rnav1"  onmouseover="sideMenu(3)" ><a href="https://2014.igem.org/Team:HIT-Harbin/Wetlab">Wetlab</a>
 
-
      <ul name="rnav">
 
-
        <li>Protocols</li>
 
-
        <li class="rfoot">Notebook</li>
 
-
    </div>
 
-
    <!--safety -->
 
-
    <div id="rsafety" class="rnav1"  onmouseover="sideMenu(4)"><a href="https://2014.igem.org/Team:HIT-Harbin/Safety">Safety</a><ul name="rnav"></ul>
 
-
    </div>
 
-
    <!--Team -->
 
-
    <div id="rteam" class="rnav1"  onmouseover="sideMenu(5)"><a href="https://2014.igem.org/Team:HIT-Harbin/Team">Team</a>
 
-
      <ul name="rnav">
 
-
        <li>Members</li>
 
-
        <li>Advisors</li>
 
-
        <li>Instructors</li>
 
-
        <li>Attribution</li>
 
-
        <li class="rfoot">Acknowledge</li>
 
-
      </ul>
 
-
    </div>
 
-
    <!--humanPractic -->
 
-
    <div id="rhumanPractic" class="rnav1" onmouseover="sideMenu(6)" ><a href="https://2014.igem.org/Team:HIT-Harbin/Humanpractic" >Human&nbsp;&nbsp;practic</a>
 
-
      <ul name="rnav">
 
-
        <li>Country Profile</li>
 
-
        <li>Qualification Trials</li>
 
-
        <li>Wechat Platform</li>
 
-
        <li>Video</li>
 
-
        <li class="rfoot">Presentation</li>
 
-
      </ul>
 
-
    </div>
 
-
  </div>
 
-
 
-
</div>
 
<script>toolbar('nav')</script>
<script>toolbar('nav')</script>

Revision as of 03:01, 11 October 2014

Parts

Sequence optimization was done using JCat(Java Codon Adaptation Tool)[1] developed by Andreas Grote

CODON OPTIMIZATION FOR HRPS AND HRPR GENE

Figure1: Codon Adaptation of hrpS gene in E.coli before optimization

CAI-Value of the sequence before optimization:0.2736. GC-Content of the sequence before optimization:59.17%

Figure2: Codon Adaptation of hrpS gene in E.coli after optimization

CAI-Value of the optimized sequence:0.9832 GC-Content of the optimized sequence:53.60%

Figure3: Codon Adaptation of hrpR gene in E.coli before optimization

CAI-Value of the sequence before optimization:0.2490. GC-Content of the sequence before optimization:57.70

Figure4: Codon Adaptation of hrpR gene in E.coli after optimization

CAI-Value of the optimized sequence:0.9721. GC-Content of the optimized sequence:53.69%

reference

[1] Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research 2005 Vol.33

Retrieved from "http://2014.igem.org/Team:HIT-Harbin/Judging"