Team:HIT-Harbin/Judging

From 2014.igem.org

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                    <div class="site-description"><h4><span class="colored"><cufon class="cufon cufon-canvas" alt="Parts" style="width: 54px; height: 20px;"><canvas width="60" height="27" style="width: 60px; height: 27px; top: -7px; left: 1px;"></canvas><cufontext>Parts</cufontext></cufon></span></h4></div>
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<p>Sequence optimization was done using JCat(Java Codon Adaptation Tool)[1] developed by  Andreas Grote </p>
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<h5 class="hashed"><span>CODON OPTIMIZATION FOR HRPS AND HRPR GENE</span></h5>
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<img src="https://static.igem.org/mediawiki/2013/f/f8/Hrps_sequence_cai_before_optimization.png">
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<p>Figure1: Codon Adaptation of hrpS gene in E.coli before optimization</p>
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<p></p>CAI-Value of the sequence before optimization:0.2736.    GC-Content of the sequence before optimization:59.17%
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<img src="https://static.igem.org/mediawiki/2013/d/d3/Hrps_sequence_cai_after_optimization.png">
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<p>Figure2: Codon Adaptation of hrpS gene in E.coli after optimization</p>
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<p></p>CAI-Value of the optimized sequence:0.9832    GC-Content of the optimized sequence:53.60%
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<img src="https://static.igem.org/mediawiki/2013/a/a8/Hrpr_sequence_cai_before_optimization.png">
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<p>Figure3: Codon Adaptation of hrpR gene in E.coli before optimization</p>
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<p></p>CAI-Value of the sequence before optimization:0.2490.    GC-Content of the sequence before optimization:57.70
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<img src="https://static.igem.org/mediawiki/2013/8/8b/Hrpr_sequence_cai_after_optimization.png">
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<p>Figure4: Codon Adaptation of hrpR gene in E.coli after optimization</p>
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<p></p>CAI-Value of the optimized sequence:0.9721.    GC-Content of the optimized sequence:53.69%
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<h4>reference</h4>
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<p></p>[1] Andreas Grote et al.  JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research 2005 Vol.33
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Revision as of 12:30, 16 August 2014

Project
  • Abstract
  • Background
  • Design
  • Modeling
  • Results
  • Future Work
Judging
  • Biobrick
  • Achievement
Wetlab
  • Protocols
  • Notebook
Team
  • Members
  • Advisors
  • Instructors
  • Attribution
  • Acknowledge
Human  practic
  • Country Profile
  • Qualification Trials
  • Wechat Platform
  • Video
  • Presentation

Parts

Sequence optimization was done using JCat(Java Codon Adaptation Tool)[1] developed by Andreas Grote

CODON OPTIMIZATION FOR HRPS AND HRPR GENE

Figure1: Codon Adaptation of hrpS gene in E.coli before optimization

CAI-Value of the sequence before optimization:0.2736. GC-Content of the sequence before optimization:59.17%

Figure2: Codon Adaptation of hrpS gene in E.coli after optimization

CAI-Value of the optimized sequence:0.9832 GC-Content of the optimized sequence:53.60%

Figure3: Codon Adaptation of hrpR gene in E.coli before optimization

CAI-Value of the sequence before optimization:0.2490. GC-Content of the sequence before optimization:57.70

Figure4: Codon Adaptation of hrpR gene in E.coli after optimization

CAI-Value of the optimized sequence:0.9721. GC-Content of the optimized sequence:53.69%

reference

[1] Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research 2005 Vol.33

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