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Team:Exeter/LabBook
From 2014.igem.org
(Difference between revisions)
| Line 78: | Line 78: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2811%29_.JPG | 11]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2811%29_.JPG | 11]]</td> | ||
<td>08/07/14</td> | <td>08/07/14</td> | ||
| - | <td></td> | + | <td>Repeat construct addition into iGEM vector and testing transformation efficiency.</td> |
<td>Edward Muir, Beth Hickton, Martyn Bennet</td> | <td>Edward Muir, Beth Hickton, Martyn Bennet</td> | ||
</tr> | </tr> | ||
| Line 84: | Line 84: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2812%29_.JPG | 12]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2812%29_.JPG | 12]]</td> | ||
<td>08/07/14</td> | <td>08/07/14</td> | ||
| - | <td></td> | + | <td>Transforming constructs into Top10 and DH5.</td> |
<td>Beth Hickton, Martyn Bennet, Edward Muir</td> | <td>Beth Hickton, Martyn Bennet, Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 90: | Line 90: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2813%29_.JPG | 13]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2813%29_.JPG | 13]]</td> | ||
<td>09/07/14</td> | <td>09/07/14</td> | ||
| - | <td></td> | + | <td>Results of Construction of iGEM vector.</td> |
<td>Jessica Rollit, Beth Hickton</td> | <td>Jessica Rollit, Beth Hickton</td> | ||
</tr> | </tr> | ||
| Line 96: | Line 96: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2814%29_.JPG | 14]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2814%29_.JPG | 14]]</td> | ||
<td>10/07/14</td> | <td>10/07/14</td> | ||
| - | <td></td> | + | <td>A range of mini-preps</td> |
<td>Jessica Rollit, Beth Hickton</td> | <td>Jessica Rollit, Beth Hickton</td> | ||
</tr> | </tr> | ||
| Line 102: | Line 102: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2815%29_.JPG | 15]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2815%29_.JPG | 15]]</td> | ||
<td>11/07/14</td> | <td>11/07/14</td> | ||
| - | <td></td> | + | <td>Measuring the length of DNA.</td> |
<td>Edward Muir</td> | <td>Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 108: | Line 108: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2816%29_.JPG | 16]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2816%29_.JPG | 16]]</td> | ||
<td>11/07/14</td> | <td>11/07/14</td> | ||
| - | <td></td> | + | <td>Measuring the length of DNA, cont.</td> |
<td>Edward Muir</td> | <td>Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 114: | Line 114: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2817%29_.JPG | 17]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2817%29_.JPG | 17]]</td> | ||
<td>11/07/14</td> | <td>11/07/14</td> | ||
| - | <td></td> | + | <td>Mini-prepping picked cultures containing promoters.</td> |
<td>Callum Bailey, Elize Hernandez</td> | <td>Callum Bailey, Elize Hernandez</td> | ||
</tr> | </tr> | ||
| Line 120: | Line 120: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2818%29_.JPG | 18]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2818%29_.JPG | 18]]</td> | ||
<td>11/07/14</td> | <td>11/07/14</td> | ||
| - | <td></td> | + | <td>Parts sent for sequencing</td> |
<td>Callum Bailey, Elize Hernandez</td> | <td>Callum Bailey, Elize Hernandez</td> | ||
</tr> | </tr> | ||
| Line 126: | Line 126: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2819%29_.JPG | 19]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2819%29_.JPG | 19]]</td> | ||
<td>11/07/14</td> | <td>11/07/14</td> | ||
| - | <td></td> | + | <td>Linearizing DNA.</td> |
<td>Edward Muir</td> | <td>Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 132: | Line 132: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2820%29_.JPG | 20]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2820%29_.JPG | 20]]</td> | ||
<td>11/07/14</td> | <td>11/07/14</td> | ||
| - | <td></td> | + | <td>Creating glycerol stocks.</td> |
<td>Beth Hickton, Max Smart</td> | <td>Beth Hickton, Max Smart</td> | ||
</tr> | </tr> | ||
| Line 138: | Line 138: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2821%29_.JPG | 21]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2821%29_.JPG | 21]]</td> | ||
<td>14/07/14</td> | <td>14/07/14</td> | ||
| - | <td></td> | + | <td>GFP and RFP resuspension and transformation.</td> |
<td>Edward Muir, Martyn Bennet, Beth Hickton</td> | <td>Edward Muir, Martyn Bennet, Beth Hickton</td> | ||
</tr> | </tr> | ||
| Line 144: | Line 144: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2822%29_.JPG | 22]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2822%29_.JPG | 22]]</td> | ||
<td>14/07/14</td> | <td>14/07/14</td> | ||
| - | <td></td> | + | <td>GFP and RFP resuspension and transformation, cont.</td> |
<td>Beth Hickton, Edward Muir</td> | <td>Beth Hickton, Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 150: | Line 150: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2823%29_.JPG | 23]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2823%29_.JPG | 23]]</td> | ||
<td>16/07/14</td> | <td>16/07/14</td> | ||
| - | <td></td> | + | <td>Mini-prepping GFP and RFP.</td> |
<td>Jessica Rollit, Edward Muir, Fran Penrose</td> | <td>Jessica Rollit, Edward Muir, Fran Penrose</td> | ||
</tr> | </tr> | ||
| Line 156: | Line 156: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2824%29_.JPG | 24]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2824%29_.JPG | 24]]</td> | ||
<td>16/07/14</td> | <td>16/07/14</td> | ||
| - | <td></td> | + | <td>GFP and RFP digestion/ligation to PSB1C3.</td> |
<td>Beth Hickton, Edward Muir</td> | <td>Beth Hickton, Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 162: | Line 162: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2825%29_.JPG | 25]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2825%29_.JPG | 25]]</td> | ||
<td>16/07/14</td> | <td>16/07/14</td> | ||
| - | <td></td> | + | <td>Plasmid digestions.</td> |
<td>Edward Muir, Callum Bailey, Benjamin Miller</td> | <td>Edward Muir, Callum Bailey, Benjamin Miller</td> | ||
</tr> | </tr> | ||
| Line 168: | Line 168: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2826%29_.JPG | 26]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2826%29_.JPG | 26]]</td> | ||
<td>16/07/14</td> | <td>16/07/14</td> | ||
| - | <td></td> | + | <td>RFP/GFP ligations.</td> |
<td>Edward Muir</td> | <td>Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 174: | Line 174: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2827%29_.JPG | 27]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2827%29_.JPG | 27]]</td> | ||
<td>16/07/14</td> | <td>16/07/14</td> | ||
| - | <td></td> | + | <td>RFP/GFP ligations, cont.</td> |
<td>Edward Muir</td> | <td>Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 180: | Line 180: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2828%29_.JPG | 28]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2828%29_.JPG | 28]]</td> | ||
<td>17/07/14</td> | <td>17/07/14</td> | ||
| - | <td></td> | + | <td>Transformation of GFP/RFP with terminator.</td> |
<td>Edward Muir</td> | <td>Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 186: | Line 186: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2829%29_.JPG | 29]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2829%29_.JPG | 29]]</td> | ||
<td>18/07/14</td> | <td>18/07/14</td> | ||
| - | <td></td> | + | <td>Making LB agar for promoters experiment.</td> |
<td>Katie Pearce</td> | <td>Katie Pearce</td> | ||
</tr> | </tr> | ||
| Line 192: | Line 192: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2830%29_.JPG | 30]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2830%29_.JPG | 30]]</td> | ||
<td>18/07/14</td> | <td>18/07/14</td> | ||
| - | <td></td> | + | <td>Promoter experiment – quantities.</td> |
<td>Edward Muir</td> | <td>Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 198: | Line 198: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2831%29_.JPG | 31]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2831%29_.JPG | 31]]</td> | ||
<td>18/07/14</td> | <td>18/07/14</td> | ||
| - | <td></td> | + | <td>Promoter experiment – quantities, cont.</td> |
<td>Edward Muir</td> | <td>Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 204: | Line 204: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2832%29_.JPG | 32]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2832%29_.JPG | 32]]</td> | ||
<td>18/07/14</td> | <td>18/07/14</td> | ||
| - | <td></td> | + | <td>Promoter experiment – quantities, cont. 2.</td> |
<td>Edward Muir</td> | <td>Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 210: | Line 210: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2833%29_.JPG | 33]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2833%29_.JPG | 33]]</td> | ||
<td>21/07/14</td> | <td>21/07/14</td> | ||
| - | <td></td> | + | <td>Promoter experiment preliminary timetable.</td> |
| - | <td></td> | + | <td>Edward Muir</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2834%29_.JPG | 34]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2834%29_.JPG | 34]]</td> | ||
<td>22/07/14</td> | <td>22/07/14</td> | ||
| - | <td></td> | + | <td>Electrophoresis of RFP and GFP (with terminators).</td> |
<td>Edward Muir</td> | <td>Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 222: | Line 222: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2835%29_.JPG | 35]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2835%29_.JPG | 35]]</td> | ||
<td>22/07/14</td> | <td>22/07/14</td> | ||
| - | <td></td> | + | <td>Preparation of competent E. coli cells.</td> |
<td>Beth Hickton, Peter Reader</td> | <td>Beth Hickton, Peter Reader</td> | ||
</tr> | </tr> | ||
| Line 228: | Line 228: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2836%29_.JPG | 36]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2836%29_.JPG | 36]]</td> | ||
<td>23/07/14</td> | <td>23/07/14</td> | ||
| - | <td></td> | + | <td>GFP/RFP + terminator liquid broths mini-prepped.</td> |
<td>Beth Hickton, Peter Reader, Jessica Rollit</td> | <td>Beth Hickton, Peter Reader, Jessica Rollit</td> | ||
</tr> | </tr> | ||
| Line 234: | Line 234: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2837%29_.JPG | 37]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2837%29_.JPG | 37]]</td> | ||
<td>23/07/14</td> | <td>23/07/14</td> | ||
| - | <td></td> | + | <td>Potassium Chromate preparation.</td> |
<td>Peter Reader, Benjamin Miller, Beth Hickton</td> | <td>Peter Reader, Benjamin Miller, Beth Hickton</td> | ||
</tr> | </tr> | ||
| Line 240: | Line 240: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2838%29_.JPG | 38]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2838%29_.JPG | 38]]</td> | ||
<td>23/07/14</td> | <td>23/07/14</td> | ||
| - | <td></td> | + | <td>Transformation of other promoters.</td> |
<td>Edward Muir</td> | <td>Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 246: | Line 246: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2839%29_.JPG | 39]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2839%29_.JPG | 39]]</td> | ||
<td>24/07/14</td> | <td>24/07/14</td> | ||
| - | <td></td> | + | <td>Moving GFP+T and RFP+T onto AMP backbones.</td> |
<td>Edward Muir, Katie Pearce</td> | <td>Edward Muir, Katie Pearce</td> | ||
</tr> | </tr> | ||
| Line 252: | Line 252: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2840%29_.JPG | 40]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2840%29_.JPG | 40]]</td> | ||
<td>24/07/14</td> | <td>24/07/14</td> | ||
| - | <td></td> | + | <td>Ligation of GFP+T and RFP+T onto AMP backbones.</td> |
<td>Edward Muir, Beth Hickton</td> | <td>Edward Muir, Beth Hickton</td> | ||
</tr> | </tr> | ||
| Line 258: | Line 258: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2841%29_.JPG | 41]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2841%29_.JPG | 41]]</td> | ||
<td>24/07/14</td> | <td>24/07/14</td> | ||
| - | <td></td> | + | <td>Potassium Dichromate colorimetry and spectrophotometry.</td> |
<td>Beth Hickton, Edward Muir</td> | <td>Beth Hickton, Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 264: | Line 264: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2842%29_.JPG | 42]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2842%29_.JPG | 42]]</td> | ||
<td>28/07/14</td> | <td>28/07/14</td> | ||
| - | <td></td> | + | <td>Plating Anderson RBSs.</td> |
<td>Beth Hickton, Edward Muir, Elize Hernandez</td> | <td>Beth Hickton, Edward Muir, Elize Hernandez</td> | ||
</tr> | </tr> | ||
| Line 270: | Line 270: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2843%29_.JPG | 43]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2843%29_.JPG | 43]]</td> | ||
<td>28/07/14</td> | <td>28/07/14</td> | ||
| - | <td></td> | + | <td>Mini-prep of reporters, promoters and RBSs.</td> |
<td>Beth Hickton, Elize Hernandez, Edward Muir</td> | <td>Beth Hickton, Elize Hernandez, Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 276: | Line 276: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2844%29_.JPG | 44]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2844%29_.JPG | 44]]</td> | ||
<td>29/07/14</td> | <td>29/07/14</td> | ||
| - | <td></td> | + | <td>COSHH Form created for Nitroglycerine experiments.</td> |
<td>Beth Hickton, Peter Reader, Benjamin Miller</td> | <td>Beth Hickton, Peter Reader, Benjamin Miller</td> | ||
</tr> | </tr> | ||
| Line 282: | Line 282: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2845%29_.JPG | 45]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2845%29_.JPG | 45]]</td> | ||
<td>30/07/14</td> | <td>30/07/14</td> | ||
| - | <td></td> | + | <td>Cr(VI) / Ligand experimentation.</td> |
<td>Peter Reader</td> | <td>Peter Reader</td> | ||
</tr> | </tr> | ||
| Line 288: | Line 288: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2846%29_.JPG | 46]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2846%29_.JPG | 46]]</td> | ||
<td>31/07/14</td> | <td>31/07/14</td> | ||
| - | <td></td> | + | <td>Mini-prep of constructs.</td> |
<td>Edward Muir, Elize Hernandez, Beth Hickton</td> | <td>Edward Muir, Elize Hernandez, Beth Hickton</td> | ||
</tr> | </tr> | ||
| Line 294: | Line 294: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2847%29_.JPG | 47]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2847%29_.JPG | 47]]</td> | ||
<td>31/07/14</td> | <td>31/07/14</td> | ||
| - | <td></td> | + | <td>Digestion and ligation of promoter/RBS combos.</td> |
<td>Edward Muir, Callum Bailey</td> | <td>Edward Muir, Callum Bailey</td> | ||
</tr> | </tr> | ||
| Line 300: | Line 300: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2848%29_.JPG | 48]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2848%29_.JPG | 48]]</td> | ||
<td>31/07/14</td> | <td>31/07/14</td> | ||
| - | <td></td> | + | <td>Oligo preparation.</td> |
<td>Edward Muir, Callum Bailey</td> | <td>Edward Muir, Callum Bailey</td> | ||
</tr> | </tr> | ||
| Line 306: | Line 306: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2849%29_.JPG | 49]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2849%29_.JPG | 49]]</td> | ||
<td>31/07/14</td> | <td>31/07/14</td> | ||
| - | <td></td> | + | <td>Ligation of promoter and RBs digestions.</td> |
<td>Edward Muir, Callum Bailey, Benjamin Miller</td> | <td>Edward Muir, Callum Bailey, Benjamin Miller</td> | ||
</tr> | </tr> | ||
| Line 312: | Line 312: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2850%29_.JPG | 50]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2850%29_.JPG | 50]]</td> | ||
<td>01/08/14</td> | <td>01/08/14</td> | ||
| - | <td></td> | + | <td>Digestion and Ligation of constructs 004 and 007.</td> |
<td>Edward Muir, Jessica Rollit, Beth Hickton</td> | <td>Edward Muir, Jessica Rollit, Beth Hickton</td> | ||
</tr> | </tr> | ||
| Line 318: | Line 318: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2851%29_.JPG | 51]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2851%29_.JPG | 51]]</td> | ||
<td>01/08/14</td> | <td>01/08/14</td> | ||
| - | <td></td> | + | <td>Digestion and Ligation of constructs 004 and 007, cont.</td> |
<td>Edward Muir</td> | <td>Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 324: | Line 324: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2852%29_.JPG | 52]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2852%29_.JPG | 52]]</td> | ||
<td>05/08/14</td> | <td>05/08/14</td> | ||
| - | <td></td> | + | <td>Colonies picked and mini-prepped.</td> |
<td>Callum Bailey</td> | <td>Callum Bailey</td> | ||
</tr> | </tr> | ||
| Line 330: | Line 330: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2853%29_.JPG | 53]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2853%29_.JPG | 53]]</td> | ||
<td>05/08/14</td> | <td>05/08/14</td> | ||
| - | <td></td> | + | <td>Colonies picked and mini-prepped, cont.</td> |
<td>Callum Bailey</td> | <td>Callum Bailey</td> | ||
</tr> | </tr> | ||
| Line 336: | Line 336: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2854%29_.JPG | 54]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2854%29_.JPG | 54]]</td> | ||
<td>06/08/14</td> | <td>06/08/14</td> | ||
| - | <td></td> | + | <td>Testing promoter/RBS combination efficiency.</td> |
<td>Callum Bailey, Edward Muir</td> | <td>Callum Bailey, Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 342: | Line 342: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2855%29_.JPG | 55]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2855%29_.JPG | 55]]</td> | ||
<td>06/08/14</td> | <td>06/08/14</td> | ||
| - | <td></td> | + | <td>Testing promoter/RBS combination efficiency, cont.</td> |
<td>Callum Bailey, Edward Muir</td> | <td>Callum Bailey, Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 348: | Line 348: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2856%29_.JPG | 56]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2856%29_.JPG | 56]]</td> | ||
<td>07/08/14</td> | <td>07/08/14</td> | ||
| - | <td></td> | + | <td>Picking colonies from promoter/RBS combination transformations. </td> |
<td>Edward Muir, Callum Bailey</td> | <td>Edward Muir, Callum Bailey</td> | ||
</tr> | </tr> | ||
| Line 354: | Line 354: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2857%29_.JPG | 57]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2857%29_.JPG | 57]]</td> | ||
<td>07/08/14</td> | <td>07/08/14</td> | ||
| - | <td></td> | + | <td>T. Cam experiment.</td> |
<td>Edward Muir, Callum Bailey</td> | <td>Edward Muir, Callum Bailey</td> | ||
</tr> | </tr> | ||
| Line 360: | Line 360: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2858%29_.JPG | 58]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2858%29_.JPG | 58]]</td> | ||
<td>11/08/14</td> | <td>11/08/14</td> | ||
| - | <td></td> | + | <td>Promoter/RBS combinations again.</td> |
<td>Edward Muir, Callum Bailey</td> | <td>Edward Muir, Callum Bailey</td> | ||
</tr> | </tr> | ||
| Line 366: | Line 366: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2859%29_.JPG | 59]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2859%29_.JPG | 59]]</td> | ||
<td>11/08/14</td> | <td>11/08/14</td> | ||
| - | <td></td> | + | <td>Promoter/RBS combinations again, cont.</td> |
<td>Edward Muir, Callum Bailey</td> | <td>Edward Muir, Callum Bailey</td> | ||
</tr> | </tr> | ||
| Line 372: | Line 372: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2860%29_.JPG | 60]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2860%29_.JPG | 60]]</td> | ||
<td>11/08/14</td> | <td>11/08/14</td> | ||
| - | <td></td> | + | <td>Inoculation of enzymes for construct 001 and 003.</td> |
<td>Edward Muir, Callum Bailey</td> | <td>Edward Muir, Callum Bailey</td> | ||
</tr> | </tr> | ||
| Line 378: | Line 378: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2861%29_.JPG | 61]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2861%29_.JPG | 61]]</td> | ||
<td>12/08/14</td> | <td>12/08/14</td> | ||
| - | <td></td> | + | <td>Inoculation of enzymes for construct 001 and 003, cont.</td> |
<td>Edward Muir, Callum Bailey</td> | <td>Edward Muir, Callum Bailey</td> | ||
</tr> | </tr> | ||
| Line 384: | Line 384: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2862%29_.JPG | 62]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2862%29_.JPG | 62]]</td> | ||
<td>12/08/14</td> | <td>12/08/14</td> | ||
| - | <td></td> | + | <td>Transformation of a sample promoter/RBS combination.</td> |
<td>Callum Bailey</td> | <td>Callum Bailey</td> | ||
</tr> | </tr> | ||
| Line 390: | Line 390: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2863%29_.JPG | 63]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2863%29_.JPG | 63]]</td> | ||
<td>12/08/14</td> | <td>12/08/14</td> | ||
| - | <td></td> | + | <td>T. Cam experiment for solvents and ether.</td> |
<td>Edward Muir</td> | <td>Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 396: | Line 396: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2864%29_.JPG | 64]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2864%29_.JPG | 64]]</td> | ||
<td>13/08/14</td> | <td>13/08/14</td> | ||
| - | <td></td> | + | <td>Combination transformation results and construct testing.</td> |
<td>Edward Muir</td> | <td>Edward Muir</td> | ||
</tr> | </tr> | ||
| Line 402: | Line 402: | ||
<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2865%29_.JPG | 65]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2865%29_.JPG | 65]]</td> | ||
<td>13/08/14</td> | <td>13/08/14</td> | ||
| - | <td></td> | + | <td>Putting oligos into GFP and RFP.</td> |
<td>Edward Muir</td> | <td>Edward Muir</td> | ||
</tr> | </tr> | ||
Revision as of 12:55, 15 August 2014
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Lab Book
On this page you can find out more about how our project progressed over time. Our data was originally written in a standard lab book. We have scanned in each page of the book. Each page is listed with along with the page number, the date the page covers, a short description of work carried out that day as well as who did it.
Page No. Date: Description: Carried out by: 1 30/06/14 Transformation of iGEM standardised DNA into plasmids for culturing. RBSs and protocols listed. Martyn Bennet, Beth Hickton 2 31/06/14 Transformation of iGEM DNA into plasmids for culturing, cont. List of promoters transformed./td> Beth Hickton, Edward Muir 3 01/07/14 List of promoters transformed, cont. Transformations were plated. Beth Hickton, Martyn Bennet 4 02/07/14 The previous day transformations were plated. Beth Hickton 5 02/07/14 Transformation of iGEM re-suspended promoters. Beth Hickton 6 02/07/14 Preparation of competent E. coli cells Beth Hickton 7 03/07/14 Promoter transformation results. Jessica Rollit, Martyn Bennet 8 03/07/14 E. coli growth rate. Edward Muir, Beth Hickton 9 04/07/14 Transformation of promoters and gene constructs. Edward Muir, Beth Hickton 10 07/07/14 Putting constructs into the iGEM vector and testing for transformation efficiency. Martyn Bennet, Edward Muir, Beth Hickton 11 08/07/14 Repeat construct addition into iGEM vector and testing transformation efficiency. Edward Muir, Beth Hickton, Martyn Bennet 12 08/07/14 Transforming constructs into Top10 and DH5. Beth Hickton, Martyn Bennet, Edward Muir 13 09/07/14 Results of Construction of iGEM vector. Jessica Rollit, Beth Hickton 14 10/07/14 A range of mini-preps Jessica Rollit, Beth Hickton 15 11/07/14 Measuring the length of DNA. Edward Muir </tr>
16 11/07/14 Measuring the length of DNA, cont. Edward Muir 17 11/07/14 Mini-prepping picked cultures containing promoters. Callum Bailey, Elize Hernandez 18 11/07/14 Parts sent for sequencing Callum Bailey, Elize Hernandez 19 11/07/14 Linearizing DNA. Edward Muir 20 11/07/14 Creating glycerol stocks. Beth Hickton, Max Smart 21 14/07/14 GFP and RFP resuspension and transformation. Edward Muir, Martyn Bennet, Beth Hickton 22 14/07/14 GFP and RFP resuspension and transformation, cont. Beth Hickton, Edward Muir 23 16/07/14 Mini-prepping GFP and RFP. Jessica Rollit, Edward Muir, Fran Penrose 24 16/07/14 GFP and RFP digestion/ligation to PSB1C3. Beth Hickton, Edward Muir 25 16/07/14 Plasmid digestions. Edward Muir, Callum Bailey, Benjamin Miller 26 16/07/14 RFP/GFP ligations. Edward Muir 27 16/07/14 RFP/GFP ligations, cont. Edward Muir 28 17/07/14 Transformation of GFP/RFP with terminator. Edward Muir 29 18/07/14 Making LB agar for promoters experiment. Katie Pearce 30 18/07/14 Promoter experiment – quantities. Edward Muir 31 18/07/14 Promoter experiment – quantities, cont. Edward Muir 32 18/07/14 Promoter experiment – quantities, cont. 2. Edward Muir 33 21/07/14 Promoter experiment preliminary timetable. Edward Muir 34 22/07/14 Electrophoresis of RFP and GFP (with terminators). Edward Muir 35 22/07/14 Preparation of competent E. coli cells. Beth Hickton, Peter Reader 36 23/07/14 GFP/RFP + terminator liquid broths mini-prepped. Beth Hickton, Peter Reader, Jessica Rollit 37 23/07/14 Potassium Chromate preparation. Peter Reader, Benjamin Miller, Beth Hickton 38 23/07/14 Transformation of other promoters. Edward Muir 39 24/07/14 Moving GFP+T and RFP+T onto AMP backbones. Edward Muir, Katie Pearce 40 24/07/14 Ligation of GFP+T and RFP+T onto AMP backbones. Edward Muir, Beth Hickton 41 24/07/14 Potassium Dichromate colorimetry and spectrophotometry. Beth Hickton, Edward Muir 42 28/07/14 Plating Anderson RBSs. Beth Hickton, Edward Muir, Elize Hernandez 43 28/07/14 Mini-prep of reporters, promoters and RBSs. Beth Hickton, Elize Hernandez, Edward Muir 44 29/07/14 COSHH Form created for Nitroglycerine experiments. Beth Hickton, Peter Reader, Benjamin Miller 45 30/07/14 Cr(VI) / Ligand experimentation. Peter Reader 46 31/07/14 Mini-prep of constructs. Edward Muir, Elize Hernandez, Beth Hickton 47 31/07/14 Digestion and ligation of promoter/RBS combos. Edward Muir, Callum Bailey 48 31/07/14 Oligo preparation. Edward Muir, Callum Bailey 49 31/07/14 Ligation of promoter and RBs digestions. Edward Muir, Callum Bailey, Benjamin Miller 50 01/08/14 Digestion and Ligation of constructs 004 and 007. Edward Muir, Jessica Rollit, Beth Hickton 51 01/08/14 Digestion and Ligation of constructs 004 and 007, cont. Edward Muir 52 05/08/14 Colonies picked and mini-prepped. Callum Bailey 53 05/08/14 Colonies picked and mini-prepped, cont. Callum Bailey 54 06/08/14 Testing promoter/RBS combination efficiency. Callum Bailey, Edward Muir 55 06/08/14 Testing promoter/RBS combination efficiency, cont. Callum Bailey, Edward Muir 56 07/08/14 Picking colonies from promoter/RBS combination transformations. Edward Muir, Callum Bailey 57 07/08/14 T. Cam experiment. Edward Muir, Callum Bailey 58 11/08/14 Promoter/RBS combinations again. Edward Muir, Callum Bailey 59 11/08/14 Promoter/RBS combinations again, cont. Edward Muir, Callum Bailey 60 11/08/14 Inoculation of enzymes for construct 001 and 003. Edward Muir, Callum Bailey 61 12/08/14 Inoculation of enzymes for construct 001 and 003, cont. Edward Muir, Callum Bailey 62 12/08/14 Transformation of a sample promoter/RBS combination. Callum Bailey 63 12/08/14 T. Cam experiment for solvents and ether. Edward Muir 64 13/08/14 Combination transformation results and construct testing. Edward Muir 65 13/08/14 Putting oligos into GFP and RFP. Edward Muir Exeter | ERASE
ERASE-The Game!
