Team:DTU-Denmark/Methods/Notebook

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<h2><span class="editsection">[<a href="/wiki/index.php?title=Team:Edinburgh/Project/Notebook&amp;action=edit&amp;section=1" title="Edit section: Chronological notebook">edit</a>]</span> <span class="mw-headline" id="Chronological_notebook"> <b>Chronological notebook</b> </span></h2>
<h2><span class="editsection">[<a href="/wiki/index.php?title=Team:Edinburgh/Project/Notebook&amp;action=edit&amp;section=1" title="Edit section: Chronological notebook">edit</a>]</span> <span class="mw-headline" id="Chronological_notebook"> <b>Chronological notebook</b> </span></h2>
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<ul><li><b>13th of June</b>:
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Participants: Casper and Kristian K
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Purpose: Preparing linearized backbone (pSB1K3) from iGEM biobrick part for USER cloning by means of PCR
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Results: Amplification was unsuccessful - no band were detected when running the PCR samples on a 1% agarose gel
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A PCR were made to amplify and attach tails for USER cloning to the linearized backbone (pSB1K3) using the primers P1 and P2. Two reactions were prepared according to the table below
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</i> We did blabla, no fluorescence.
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<ul><li><b>14th of June</b>:
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Participants:
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Purpose: Investigating the success of the PCR the day before by gel electrophoresis
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Results: PCR amplification were unsuccessful - no bands were detected
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PCR products from 130614 was run on a 1% agarose + 1X TAE buffer GEL.
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5ul PCR product + 1ul loading dye was loaded on the gel as well as 4ul ladder 1 kb.
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Revision as of 12:43, 15 August 2014

Technical University of Denmark

Notebook


[edit] Chronological notebook

  • 13th of June:

Participants: Casper and Kristian K Purpose: Preparing linearized backbone (pSB1K3) from iGEM biobrick part for USER cloning by means of PCR Results: Amplification was unsuccessful - no band were detected when running the PCR samples on a 1% agarose gel A PCR were made to amplify and attach tails for USER cloning to the linearized backbone (pSB1K3) using the primers P1 and P2. Two reactions were prepared according to the table below We did blabla, no fluorescence.

  • 14th of June:

Participants: Purpose: Investigating the success of the PCR the day before by gel electrophoresis Results: PCR amplification were unsuccessful - no bands were detected PCR products from 130614 was run on a 1% agarose + 1X TAE buffer GEL. 5ul PCR product + 1ul loading dye was loaded on the gel as well as 4ul ladder 1 kb.

  • 27th of June:

Fluorescence We did blabla, no fluorescence.

  • 1st of July:

Headline EtOH resistance assay was continued in a narrow concentration range (Jan).

E. coli (DH5alpha) cells were transformed using BioBricks required for EtOH production (Aleksandra).

O/N cultures of E. coli and B. subtilis were set up to continue with the experiments tomorrow (Harry).

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