Team:Evry/Notebook/CellCharacterization/Antibiotic test

From 2014.igem.org

(Difference between revisions)
(Created page with "<html> <head> </head> <body> <style type="text/css">.conc { text-align: center } .title2 { text-align: center; font-family: calibri } .title4 { font-family: calibri }</style> <d...")
Line 30: Line 30:
<tr>
<tr>
   <td> Amplicilin </td>
   <td> Amplicilin </td>
-
   <td class= conc> 100 mg/mL </td>
+
   <td class= conc> 50 mg/mL </td>
   <td> Weight 1g of Amplicilin powder, solubilization into 40mL of miliQ water. <br>Vortex 5min and filtration with a 200nm filter. </br>  Stock : -20°C (aliquots of 1mL) </td>
   <td> Weight 1g of Amplicilin powder, solubilization into 40mL of miliQ water. <br>Vortex 5min and filtration with a 200nm filter. </br>  Stock : -20°C (aliquots of 1mL) </td>
</tr>
</tr>

Revision as of 16:04, 14 August 2014

Antibiotic test

Preparation of antibiotic stocks

Antibiotic Stock concentration Protocol
Kanamycin 25 mg/mL Weight 0.56g of Kanamycin powder, solubilization into 20mL of miliQ water.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C (aliquots of 1mL)
Streptomycin 100 mg/mL Weight 1g of Streptomycin powder, solubilization into 20mL of miliQ water.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C (aliquots of 1mL)
Amplicilin 50 mg/mL Weight 1g of Amplicilin powder, solubilization into 40mL of miliQ water.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C (aliquots of 1mL)
Tetracyclin 15 mg/mL Weight 0.6g of Tetracyclin powder, solubilization into 20mL of ethanol 50%.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C and hiden from light(aliquots of 1mL)
Chloramphenicol 34 mg/mL Weight 0.34g of Chloramphenicol powder, solubilization into 10mL of ethanol 100%.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C and hiden from light(aliquots of 1mL)