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Team:Evry/Notebook/CellCharacterization
From 2014.igem.org
(Difference between revisions)
(Preparation of antibiotic stocks) |
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| - | < | + | <h2 class=title2> <b> Antibiotic test </b></h2> |
| - | <div class=" | + | <div class="CellCharacterization"> |
| - | + | <h4 class=title4> <u> Preparation of antibiotic stocks </u> </h4> | |
<p> | <p> | ||
| + | <table> | ||
| + | <tr class = conc> | ||
| + | |||
| + | <td> <b>Antibiotic </b> </td> | ||
| + | <td> <b>Stock concentration </b> </td> | ||
| + | <td> <b>Protocol </b> </td> | ||
| + | </tr> | ||
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| + | |||
| + | <tr> | ||
| + | <td>Kanamycin </td> | ||
| + | <td class= conc> 25 mg/mL </td> | ||
| + | <td> Weight 0.56g of Kanamycin powder, solubilization into 20mL of miliQ water. <br>Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C (aliquots of 1mL)</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td> Streptomycin </td> | ||
| + | <td class= conc> 100 mg/mL </td> | ||
| + | <td> Weight 1g of Streptomycin powder, solubilization into 20mL of miliQ water.<br> Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C (aliquots of 1mL) </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td> Amplicilin </td> | ||
| + | <td class= conc> 100 mg/mL </td> | ||
| + | <td> Weight 1g of Amplicilin powder, solubilization into 40mL of miliQ water. <br>Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C (aliquots of 1mL) </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td> Tetracyclin</td> | ||
| + | <td class= conc> 15 mg/mL </td> | ||
| + | <td> Weight 0.6g of Tetracyclin powder, solubilization into 20mL of ethanol 50%. <br>Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C and hiden from light(aliquots of 1mL) </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td> Chloramphenicol </td> | ||
| + | <td class= conc> 34 mg/mL </td> | ||
| + | <td> Weight 0.34g of Chloramphenicol powder, solubilization into 10mL of ethanol 100%. <br> Vortex 5min and filtration with a 200nm filter. </br> Stock : -20°C and hiden from light(aliquots of 1mL) </td> | ||
| + | </tr> | ||
| + | |||
| + | </table> | ||
| - | |||
</p> | </p> | ||
Revision as of 15:45, 14 August 2014
Antibiotic test
Preparation of antibiotic stocks
| Antibiotic | Stock concentration | Protocol |
| Kanamycin | 25 mg/mL | Weight 0.56g of Kanamycin powder, solubilization into 20mL of miliQ water. Vortex 5min and filtration with a 200nm filter. Stock : -20°C (aliquots of 1mL) |
| Streptomycin | 100 mg/mL | Weight 1g of Streptomycin powder, solubilization into 20mL of miliQ water. Vortex 5min and filtration with a 200nm filter. Stock : -20°C (aliquots of 1mL) |
| Amplicilin | 100 mg/mL | Weight 1g of Amplicilin powder, solubilization into 40mL of miliQ water. Vortex 5min and filtration with a 200nm filter. Stock : -20°C (aliquots of 1mL) |
| Tetracyclin | 15 mg/mL | Weight 0.6g of Tetracyclin powder, solubilization into 20mL of ethanol 50%. Vortex 5min and filtration with a 200nm filter. Stock : -20°C and hiden from light(aliquots of 1mL) |
| Chloramphenicol | 34 mg/mL | Weight 0.34g of Chloramphenicol powder, solubilization into 10mL of ethanol 100%. Vortex 5min and filtration with a 200nm filter. Stock : -20°C and hiden from light(aliquots of 1mL) |
