Team:ITB Indonesia/Project

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<p> PET or commonly known as plastic has been widely used ranging from domestic to industry. We can’t deny that plastic gives us benefits. Despite a lot of benefits that we can take, the overproduction of plastic has serious impact on the environment if not treated properly. Increasing 7% global plastic production annually has been estimated to likely exceed 24.39 million tons in 2015. Appropriate treatment is needed, otherwise plastic abundant will be treathening surroundings, the life of animal and human beings. As we know that PET will take 500 or even 1000 years to degrade by process called photodegradation rather than biodegradation, thus making a necessity to treat plastic such as degradation or recycling. Degradation by burning or using chemical methode will also bring harmfull effect. Recycling is jut an alternative that won’t overcome this problem completely, while reuse seems inappropriate to do due to plastic age. When it’s reused severe health complication may occur.  
<p> PET or commonly known as plastic has been widely used ranging from domestic to industry. We can’t deny that plastic gives us benefits. Despite a lot of benefits that we can take, the overproduction of plastic has serious impact on the environment if not treated properly. Increasing 7% global plastic production annually has been estimated to likely exceed 24.39 million tons in 2015. Appropriate treatment is needed, otherwise plastic abundant will be treathening surroundings, the life of animal and human beings. As we know that PET will take 500 or even 1000 years to degrade by process called photodegradation rather than biodegradation, thus making a necessity to treat plastic such as degradation or recycling. Degradation by burning or using chemical methode will also bring harmfull effect. Recycling is jut an alternative that won’t overcome this problem completely, while reuse seems inappropriate to do due to plastic age. When it’s reused severe health complication may occur.  
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Based on this case, we want to diversify the methode on treating plastic overproduction using synthetic biological approach. We create novel device which is synthetic bacteria that can degrade PET and also consume the product of degradation reaction ( ethylene glycol ). We establish ompA fused with LC Cutinase on E.coli BL21 giving rise to displaying enzymes on the surface of bacteria. Thus making ease on the degradation process which substrate ( in this case PET ) with high molecular weight doen’t need to accross cell membrane. We also construct a casette comprised of two enzymes, Glycoaldehide reductase and glycoaldehide dehidrogenase which responsible to metabolize ethylene glycol. We use constitutive promoter for both  construct. Thus making us necessery to create a module of reporter and self regulatory in order to make system is efficiently to run and minimizing severe cytoplasmic stress, in this case inclusion body aggregate due to strong constitutive expression.
Based on this case, we want to diversify the methode on treating plastic overproduction using synthetic biological approach. We create novel device which is synthetic bacteria that can degrade PET and also consume the product of degradation reaction ( ethylene glycol ). We establish ompA fused with LC Cutinase on E.coli BL21 giving rise to displaying enzymes on the surface of bacteria. Thus making ease on the degradation process which substrate ( in this case PET ) with high molecular weight doen’t need to accross cell membrane. We also construct a casette comprised of two enzymes, Glycoaldehide reductase and glycoaldehide dehidrogenase which responsible to metabolize ethylene glycol. We use constitutive promoter for both  construct. Thus making us necessery to create a module of reporter and self regulatory in order to make system is efficiently to run and minimizing severe cytoplasmic stress, in this case inclusion body aggregate due to strong constitutive expression.
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Revision as of 11:40, 14 August 2014



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Project Description

Content

PET or commonly known as plastic has been widely used ranging from domestic to industry. We can’t deny that plastic gives us benefits. Despite a lot of benefits that we can take, the overproduction of plastic has serious impact on the environment if not treated properly. Increasing 7% global plastic production annually has been estimated to likely exceed 24.39 million tons in 2015. Appropriate treatment is needed, otherwise plastic abundant will be treathening surroundings, the life of animal and human beings. As we know that PET will take 500 or even 1000 years to degrade by process called photodegradation rather than biodegradation, thus making a necessity to treat plastic such as degradation or recycling. Degradation by burning or using chemical methode will also bring harmfull effect. Recycling is jut an alternative that won’t overcome this problem completely, while reuse seems inappropriate to do due to plastic age. When it’s reused severe health complication may occur. Based on this case, we want to diversify the methode on treating plastic overproduction using synthetic biological approach. We create novel device which is synthetic bacteria that can degrade PET and also consume the product of degradation reaction ( ethylene glycol ). We establish ompA fused with LC Cutinase on E.coli BL21 giving rise to displaying enzymes on the surface of bacteria. Thus making ease on the degradation process which substrate ( in this case PET ) with high molecular weight doen’t need to accross cell membrane. We also construct a casette comprised of two enzymes, Glycoaldehide reductase and glycoaldehide dehidrogenase which responsible to metabolize ethylene glycol. We use constitutive promoter for both construct. Thus making us necessery to create a module of reporter and self regulatory in order to make system is efficiently to run and minimizing severe cytoplasmic stress, in this case inclusion body aggregate due to strong constitutive expression.


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