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Team:Paris Bettencourt/Notebook/Old People Smell
From 2014.igem.org
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<li> Water: bring to 1L</li> | <li> Water: bring to 1L</li> | ||
</ul> | </ul> | ||
| + | |||
| + | </br> | ||
| + | <p id="text"><u> 3) M9 Minimal Media 100% 2-nonenal</u></p> | ||
| + | <ul> | ||
| + | <li>200mL M9 Salts</li> | ||
| + | <li>0.1mL CaCl2 1M</li> | ||
| + | <li>2mL MgSO4 1M</li> | ||
| + | <li>20mL 2-nonenal 10,7%*</li> | ||
| + | <li> Water: bring to 1L</li> | ||
| + | </ul> | ||
| + | |||
| + | <p>We autoclaved 6 bottles (2 of 500mL for each)</p> | ||
| + | <p>Then, we made liquid culture of C.striatum in media <b>1)</b> to try if the minima media works.</p> | ||
| + | |||
| + | |||
<h5 id="date">Date 2</h5> | <h5 id="date">Date 2</h5> | ||
Revision as of 09:32, 14 August 2014
Paris Bettencourt 2014
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Notebook
Old Person odor
August
August 5th 1
The goal is to select a bacteria able to develop on 2-nonenal. We made 2 new media:
1) M9 Minimal Media 100% Glucose
- 200mL M9 Salts
- 0.1mL CaCl2 1M
- 2mL MgSO4 1M
- 20mL Glucose 20%
- Water: bring to 1L
2) M9 Minimal Media 50% Glucose - 50% 2-Nonenal (according to the number of carbon atoms)
- 200mL M9 Salts
- 0.1mL CaCl2 1M
- 2mL MgSO4 1M
- 10mL Glucose 20%
- 10mL 2-nonenal 10,7%*
- Water: bring to 1L
3) M9 Minimal Media 100% 2-nonenal
- 200mL M9 Salts
- 0.1mL CaCl2 1M
- 2mL MgSO4 1M
- 20mL 2-nonenal 10,7%*
- Water: bring to 1L
We autoclaved 6 bottles (2 of 500mL for each)
Then, we made liquid culture of C.striatum in media 1) to try if the minima media works.
Date 2
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September
Date 1
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Date 2
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October
Date 1
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Date 2
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