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Team:Gothenburg/Calendar
From 2014.igem.org
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<td id="2014-05-29" class="date prev-month"> | <td id="2014-05-29" class="date prev-month"> | ||
<h1>29</h1> | <h1>29</h1> | ||
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<td id="2014-06-03" class="date"> | <td id="2014-06-03" class="date"> | ||
<h1>3</h1> | <h1>3</h1> | ||
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<td id="2014-06-04" class="date"> | <td id="2014-06-04" class="date"> | ||
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<td id="2014-06-05" class="date"> | <td id="2014-06-05" class="date"> | ||
<h1>5</h1> | <h1>5</h1> | ||
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<td id="2014-06-06" class="date"> | <td id="2014-06-06" class="date"> | ||
<h1>6</h1> | <h1>6</h1> | ||
<ul class="titles"> | <ul class="titles"> | ||
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<td id="2014-06-07" class="date"> | <td id="2014-06-07" class="date"> | ||
<h1>7</h1> | <h1>7</h1> | ||
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<td id="2014-06-10" class="date"> | <td id="2014-06-10" class="date"> | ||
<h1>10</h1> | <h1>10</h1> | ||
<ul class="titles"> | <ul class="titles"> | ||
| - | <li> | + | <li>Safety Instructions</li> |
| - | + | ||
</ul> | </ul> | ||
| - | <span class="hidden"> | + | <span class="hidden"><p> The team received mandatory safety training and general lab routine information. |
| + | <br> <p> A really cool video about Chalmers' chemical safety routine (recorded at our very own lab!) can be seen in http://www.chalmers.se/insidan/EN/about-chalmers/environmental-work/policies/chemical-safety-routine</span> | ||
</td> | </td> | ||
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<ul class="titles"> | <ul class="titles"> | ||
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<ul class="titles"> | <ul class="titles"> | ||
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<ul class="titles"> | <ul class="titles"> | ||
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<h1>16</h1> | <h1>16</h1> | ||
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<h1>17</h1> | <h1>17</h1> | ||
<ul class="titles"> | <ul class="titles"> | ||
| - | <li> | + | <li>Introduction to centrifuges and autoclaves</li> |
| - | + | ||
</ul> | </ul> | ||
| - | <span class="hidden"> | + | <span class="hidden"> |
| + | <p> As a part of the mandatory training required by the SysBio group, the team received instructions on how to operate the centrifuges | ||
| + | and autoclaves of the labs. | ||
| + | <br><p>We also worked on our risk declaration; a mandatory document containing our experiments descriptions and information about the chemicals we are going to use, their handling, storage and associated risk, as well as waste handling.</span> | ||
</td> | </td> | ||
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<h1>18</h1> | <h1>18</h1> | ||
<ul class="titles"> | <ul class="titles"> | ||
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<h1>19</h1> | <h1>19</h1> | ||
<ul class="titles"> | <ul class="titles"> | ||
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<h1>20</h1> | <h1>20</h1> | ||
<ul class="titles"> | <ul class="titles"> | ||
| - | <li> | + | <li>Finished mandatory training</li> |
| - | + | ||
</ul> | </ul> | ||
| - | <span class="hidden"> | + | <span class="hidden"> |
| + | <p>Our Risk Declaration was approved by the lab manager and our supervisors. We also sign a safety certificate stating that we are aware | ||
| + | of the safety instructions and with that, completed the mandatory "check-in list for new incomers" of the lab.</span> | ||
</td> | </td> | ||
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<h1>21</h1> | <h1>21</h1> | ||
<ul class="titles"> | <ul class="titles"> | ||
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<h1>22</h1> | <h1>22</h1> | ||
<ul class="titles"> | <ul class="titles"> | ||
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<h1>23</h1> | <h1>23</h1> | ||
<ul class="titles"> | <ul class="titles"> | ||
| - | <li> | + | <li>Media Preparation</li> |
| - | + | ||
</ul> | </ul> | ||
| - | <span class="hidden"> | + | <span class="hidden"> |
| + | <p>On the first day of laboration, we prepared LB, YPD and SC-Ura/His/Trp-Leu and YPD media. | ||
| + | <br>Click here to see the protocol. [[PUT METHOD]]! | ||
| + | <br>[[File:20140623_103124.jpg]],[[File:20140623_115809.jpg|frameless|300px]]</span> | ||
</td> | </td> | ||
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<h1>24</h1> | <h1>24</h1> | ||
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<h1>25</h1> | <h1>25</h1> | ||
<ul class="titles"> | <ul class="titles"> | ||
| - | <li> | + | <li>Amplification and Purification of non-synthetic parts</li> |
| - | + | ||
</ul> | </ul> | ||
| - | <span class="hidden"> | + | <span class="hidden"> |
| + | <p> Succesfully PCR amplified five out of the seven parts from yeast genome via a Phusion High-Fidelity DNA Polymerase. | ||
| + | <br>Click here to see the protocol. [[PUT METHOD]]!<br> | ||
| + | <p>The PCR program used was: <br> | ||
| + | <ol> | ||
| + | <li>3 min at 98ºC </li> | ||
| + | <li>10s at 98ºC </li> | ||
| + | <li>Gradient from 52 to 61ºC for 30s </li> | ||
| + | <li>1 min at 72ºC </li> | ||
| + | <li>Go to step 2 (repeat 39 times) </li> | ||
| + | <li>10 min at 72ºC. </li> | ||
| + | </ol> | ||
| + | <p>A diagnostic gel was performed and the result was as follows: | ||
| + | [[File: Bio-Rad 2014-06-25 15hr 34min.jpg|frameless|300px]] <br> | ||
| + | <p>The purification of the succesfully amplified parts was done using a PCR Purification Kit from GenJet following the instructions | ||
| + | of the manufacture's manual but with no addition of isopropanol. <br> | ||
| + | </span> | ||
</td> | </td> | ||
Revision as of 12:02, 14 August 2014
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The team received mandatory safety training and general lab routine information.
A really cool video about Chalmers' chemical safety routine (recorded at our very own lab!) can be seen in http://www.chalmers.se/insidan/EN/about-chalmers/environmental-work/policies/chemical-safety-routine |
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As a part of the mandatory training required by the SysBio group, the team received instructions on how to operate the centrifuges
and autoclaves of the labs.
We also worked on our risk declaration; a mandatory document containing our experiments descriptions and information about the chemicals we are going to use, their handling, storage and associated risk, as well as waste handling. |
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Our Risk Declaration was approved by the lab manager and our supervisors. We also sign a safety certificate stating that we are aware of the safety instructions and with that, completed the mandatory "check-in list for new incomers" of the lab. |
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On the first day of laboration, we prepared LB, YPD and SC-Ura/His/Trp-Leu and YPD media.
|
24 |
25
Succesfully PCR amplified five out of the seven parts from yeast genome via a Phusion High-Fidelity DNA Polymerase.
The PCR program used was:
A diagnostic gel was performed and the result was as follows:
[[File: Bio-Rad 2014-06-25 15hr 34min.jpg|frameless|300px]] The purification of the succesfully amplified parts was done using a PCR Purification Kit from GenJet following the instructions
of the manufacture's manual but with no addition of isopropanol. |
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