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| - | <section style="text-align: right; margin: 50px">
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| - | <i>A selection of results obtained by our methods will be shown in the next section</i>.</p> </section>
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| - | <section style="text-align: right; margin: -75px"><p>
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| - | ¨ </section>
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| - | <section style="text-align: justify; margin: 125px">
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| - | <section style="text-align: center; margin: 30px">
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| - | <h3>Material</h3></section>
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| - | <section style="text-align: justify; margin: 0px">
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| - | A list of the Material is not yet accessible, but we hope this will be soon written. The usual equipment was:
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| - | gloves, glasses and coat (especially because of UV emission & Ethidium Bromide during electrophoresis).
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| - | There's more information in the page related with Safety.</p>
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| - | ________________________________________________________________________________________________________________
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| - | </p> </section>
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| - | <section style="text-align: center; margin: 30px">
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| - | <h3>Methods</h3></section>
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| - | <section style="text-align: justify; margin: 0px">
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| - | <p>First, birth and growing of bacteria populations (Centrifugation, Dilution).</p>
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| - | <p>Secondly, insertion of the biobricks and plasmids chosen with E. Coli (Electroporation method, PCR Amplification, Electrophoresis).
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| - | Just after this step, bacteria selection (in function of the quantities od oligopeptids or phoA+prolin) and inclusion of a toxin-antitoxin (TA: ccdB-ccdA) system in E. Coli.</p>
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| - | <p>Then, addiction of fluorescent proteins (GFP, RFP) and determination of the quantities and the principal properties of our bacteria
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| - | (including Emission Spectroscopy) and analize of their genetical sequences.
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| - | <p>Finally, conservation of bacteria populations producting the desired molecules or proteins in good quantities, at cold temperature in petri boxes and containers.</p></section>
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| - | </section>
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| - | <section style="text-align: right; margin: -50px"><p>
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| - | ¨ </section>
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| | <tr><td><br/><br/></td></tr> | | <tr><td><br/><br/></td></tr> |
| | </table> | | </table> |
| | </div> | | </div> |
$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
\newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}}
\newcommand{\Stabi}{\small Stabi}$
$\newcommand{\EColi}{\small E.coli}
\newcommand{\SCere}{\small S.cerevisae}\\[0cm]
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
\newcommand{\PI}{\small PI}$
$\newcommand{\Igo}{\Large\mathcal{I}}
\newcommand{\Tgo}{\Large\mathcal{T}}
\newcommand{\Ogo}{\Large\mathcal{O}}
~$
"
- Université Libre de Bruxelles -
