Team:UT-Dallas/Notebook/8-05

From 2014.igem.org

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Transform.
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List out mis-match of gRNA&reporter vectors.
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Inoculate reporter vector from glycerol stock.
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%http://i.imgur.com/vbcnl2B.jpg
%http://i.imgur.com/vbcnl2B.jpg
Test digest for reporter vectors August 5
Test digest for reporter vectors August 5
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<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
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<h1 class="firstHeading" align="center">Tuesday, August 05, 2014</h1>
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<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-11&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
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<p class="tab">
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  We started using Kristina home stock cell now instead of our own cell and the commercial cells.</p>
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<p class="tab">
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  We have a set of reporter vectors that we grew and harvested the DNA last week. Today, to qualitatively check that we got what we want, we are going to test digest our reporter vectors. After the check, we will send our second batch of sequencing order.</p>
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<p class="tab">Kristina brought lots of food: cookies, muffins, etc.
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      <th><font size="3">Today's tasks:</font>
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<LI> Transform.
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<LI> List out mis-match of gRNA&reporter vectors.
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        <LI> Inoculate reporter vector from glycerol stock.
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      <img src="<---------image from today----------->" height="400px">
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      <br> Preparing cells for plasmid purification.
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      <img src="<---------image from today----------->" style="border-width: 0px; display: inline; margin-left: 0px; margin-top: 0px;" height="400px">
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<br> Description of image from today
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Revision as of 19:44, 11 August 2014

%http://i.imgur.com/vbcnl2B.jpg

Test digest for reporter vectors August 5 %http://i.imgur.com/s2bH0st.jpg

Tuesday, August 05, 2014

Click here to edit the page

We started using Kristina home stock cell now instead of our own cell and the commercial cells.

We have a set of reporter vectors that we grew and harvested the DNA last week. Today, to qualitatively check that we got what we want, we are going to test digest our reporter vectors. After the check, we will send our second batch of sequencing order.

Kristina brought lots of food: cookies, muffins, etc.

Today's tasks:
  • Transform.
  • List out mis-match of gRNA&reporter vectors.
  • Inoculate reporter vector from glycerol stock.


Preparing cells for plasmid purification.


Description of image from today

Retrieved from "http://2014.igem.org/Team:UT-Dallas/Notebook/8-05"