Team:Paris Saclay/Notebook/August/8

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(Difference between revisions)
(→Friday 8th August)
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===D - Lemon Scent===
===D - Lemon Scent===
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We have done PCR with pcola (geranyl synthase) and BBa517003:
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''by Mélanie & Pierre''
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 +
 
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We have done a PCR with pcola (geranyl synthase) and BBa_K517003:
Green GotaqBuffer = 10µl
Green GotaqBuffer = 10µl
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Oligo used : iPS81bis AND iPS82 (pcola)      iPS69 and iPS68bis (BBa517003)
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Oligo used : iPS81bis AND iPS82 (pcola)      iPS69 and iPS68bis (BBa_K517003)
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PCR cycle:
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PCR cycles:
95° 2min
95° 2min
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71° 5min
71° 5min
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 +
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Electrophoresis to test results :
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 +
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#10 µl of '''BBa_K517003'''
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#10 µl of '''BBa_K517003'''
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#10 µl of '''BBa_K517003'''
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#10 µl of '''BBa_K517003'''
 +
#10 µl of '''BBa_K517003'''
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#10 µl of '''p. cola'''
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#10 µl of '''p. cola'''
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#10 µl of '''p. cola'''
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#10 µl of '''p. cola'''
 +
#10 µl of '''p. cola'''
 +
 +
 +
 +
Purification of the previous PCR
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 +
''by Pierre''
 +
 +
From the 5 tubes of p.Cola I purified two tubes.
 +
From the 5 tubes of BBa_K517003 I purified two tubes.
 +
 +
I did an electroforesis to check if the purification has worked. It did.
 +
 +
#1 µl of '''BBa_K517003'''
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#1 µl of '''p. cola'''
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 +
====stock of pcola plasmide====
====stock of pcola plasmide====
using the DNA kit extraction to extract pcola ()
using the DNA kit extraction to extract pcola ()
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==Human Pratices==
==Human Pratices==

Revision as of 17:15, 8 August 2014

Contents

Friday 8th August

Lab work

D - Lemon Scent

by Mélanie & Pierre


We have done a PCR with pcola (geranyl synthase) and BBa_K517003:

Green GotaqBuffer = 10µl

MgCl2 = 4µl

dNTP = 1µl

Primer A = 1µM

Primer B = 1µM

DNA = 1µl

Gotaq DNA polymerase = 0.25µl


Oligo used : iPS81bis AND iPS82 (pcola) iPS69 and iPS68bis (BBa_K517003)

PCR cycles:

95° 2min

95° 30sec

49-55° 30 sec

72° 2min

71° 5min


Electrophoresis to test results :


  1. 10 µl of BBa_K517003
  2. 10 µl of BBa_K517003
  3. 10 µl of BBa_K517003
  4. 10 µl of BBa_K517003
  5. 10 µl of BBa_K517003
  6. 10 µl of p. cola
  7. 10 µl of p. cola
  8. 10 µl of p. cola
  9. 10 µl of p. cola
  10. 10 µl of p. cola


Purification of the previous PCR

by Pierre

From the 5 tubes of p.Cola I purified two tubes. From the 5 tubes of BBa_K517003 I purified two tubes.

I did an electroforesis to check if the purification has worked. It did.

  1. 1 µl of BBa_K517003
  2. 1 µl of p. cola



stock of pcola plasmide

using the DNA kit extraction to extract pcola ()

Human Pratices

Art & Design

by Terry

Sequel of yesterday molding attempt.

The mold is cut delicately and the test piece removed.

Photo agar test 003.jpg

Then, the mold is placed back in the becher and filled up with regular agar ( 20mg/ml )

Result : FAILURE, the agar test piece has been ripped when unmolding. It was stuck to the mold.

But the mold is still intact ! I cleaned it up and slightly covered the inward surface with liquid soap. The mold is filled up again and conserved in the refrigerator for 2 hours.

Result : SUCCESS, I slightly remoistened the gel with water for an easier unmolding. The agar test piece is perfectly removed.

Photo agar test 004.jpg

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