Safety/White List

From 2014.igem.org

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<p>Ask us! Contact safety AT igem DOT org. Alternatively, because the Check-In form is short, you could choose to send a Check-In even if you are unsure.</p>
<p>Ask us! Contact safety AT igem DOT org. Alternatively, because the Check-In form is short, you could choose to send a Check-In even if you are unsure.</p>
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<p><em>What exactly counts as a "whole organism"?</em></p>
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<p>For the purposes of this White List, a "whole organism" is an entire cell or multicellular organism, whether alive or dead. Intact, isolated viral genomes are also considered "whole organism", because many viral genomes can be pathogenic if they enter a host cell, even without the viral capsule. (Isolated non-viral genomes are considered parts.)</p>
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<p><em>We are going to handle an organism in lab, but we will only extract some DNA from it using PCR -- we won't use it as our chassis. Do we still have to send a Check-In?</em></p>
<p><em>We are going to handle an organism in lab, but we will only extract some DNA from it using PCR -- we won't use it as our chassis. Do we still have to send a Check-In?</em></p>
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<p>Yes. The Check-In requirement applies to all organisms and all parts that you will handle in the lab, even if they will not be part of your final project.</p>
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<p><em>Our project is to detect a dangerous organism. In order to test our project, of course we need to handle the dangerous organism (or parts of it), but it will not be part of what we build. Do we still have to send a Check-In?</em></p>
<p><em>Our project is to detect a dangerous organism. In order to test our project, of course we need to handle the dangerous organism (or parts of it), but it will not be part of what we build. Do we still have to send a Check-In?</em></p>

Revision as of 20:52, 12 May 2014


Questions or feedback?
Email safety AT igem DOT org!

PAGE UNDER CONSTRUCTION -- NOT READY FOR USE

This White List is a draft. It is not the final version!

Questions? Contact

  1. Obtain Claudia's feedback on white list
  2. Consult VFDB / protein taxonomies
  3. Format nicely and post
  4. Write FAQ
  5. Remove this note.


Remember!

You must Check-In for ANY organism or part that is NOT on the White List. The right-hand column of the table below is only examples of organisms/parts that require a Check-In. If your part/organism is not in the table below, it still requires a Check-In.

Questions? Contact



White List (no Check-In required) Check-In Required
Whole Organisms
  • Risk Group 1 microorganisms
  • Risk Group 2 microorganisms
  • Human and primate cell lines that have been tested and certified free of known pathogens (consult your vendor)
  • Cell lines from plants, fungi, or animals that are not primates
  • Human and primate cell lines that may contain pathogens
  • All primary isolated cells
  • C. elegans, Physcomitrella patens, Drosophila spp.
  • Human subjects in non-invasive experiments, such as surveys (see note at bottom of page)
  • Other multicellular organisms
  • ...and anything not explicitly listed
Parts
  • Any part from a Risk Group 1 organism, regardless of its function
  • All Registry parts, except those flagged by Archetype or Safety Committee
  • Non-protein-coding parts in the following categories:
    • Promoters, RBSes, Terminators
    • Binding sites for transcriptional regulators, endonucleases, and other proteins that bind to DNA
    • CRISPR guide RNAs, microRNAs / interfering RNAs, aptamers
  • Protein-coding genes from animals, plants, or Risk Group 2 microorganisms, in the following categories:
    • Category 1
    • Category 2
  • Any part from a Risk Group 3 or 4 organism, regardless of its function
  • Registry parts with red flags on
  • Non-protein-coding genes that do not fall in any of the white-listed categories
  • Protein-coding genes from animals, plants, or Risk Group 2 microorganisms, that do not fall in any of the white-listed categories


Banned Organisms/Parts

Some of the most dangerous organisms and parts are not allowed in iGEM, even with a Check-In:

  • Whole organisms from Risk Group 3
  • Whole organisms from Risk Group 4
  • Parts from Risk Group 4 organisms

If you find that you want to use a banned organism/part, you should redesign your project to use a substitute from a safer Risk Group. Consult your advisor or contact us at to get some advice on choosing a substitute.

Notes

  • What if I'm not sure whether my organism/part requires a Check-In?

    Ask us! Contact safety AT igem DOT org. Alternatively, because the Check-In form is short, you could choose to send a Check-In even if you are unsure.

  • What exactly counts as a "whole organism"?

    For the purposes of this White List, a "whole organism" is an entire cell or multicellular organism, whether alive or dead. Intact, isolated viral genomes are also considered "whole organism", because many viral genomes can be pathogenic if they enter a host cell, even without the viral capsule. (Isolated non-viral genomes are considered parts.)

  • We are going to handle an organism in lab, but we will only extract some DNA from it using PCR -- we won't use it as our chassis. Do we still have to send a Check-In?

    Yes. The Check-In requirement applies to all organisms and all parts that you will handle in the lab, even if they will not be part of your final project.

  • Our project is to detect a dangerous organism. In order to test our project, of course we need to handle the dangerous organism (or parts of it), but it will not be part of what we build. Do we still have to send a Check-In?

    Yes. The Check-In requirement applies to all organisms and all parts that you will handle in the lab, even if they will not be part of your final project.

  • What about experiments on human subjects, such as surveys or software user-testing?

    Human experimental subjects are not covered under the iGEM safety program. If you conduct any experiments with human subjects (including non-invasive work such as surveys or software user-testing), you must find out the rules and regulations that cover those experiments in your country / at your university. Some experiments and surveys might require the approval of a review board on human experimental subjects, and some might not require any approval.

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