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Team:NTNU Trondheim/Notebook
From 2014.igem.org
(Difference between revisions)
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<div class="nb-onetech-i nohilite">show technical details</div> | <div class="nb-onetech-i nohilite">show technical details</div> | ||
</h6> | </h6> | ||
| - | + | <div class="nb-tech">testtesttesttest test</div> | |
<p> <ol> | <p> <ol> | ||
<li>Prepared SOC and yB solutions for future lab work.</li> | <li>Prepared SOC and yB solutions for future lab work.</li> | ||
<li>Sterilized material and solutions needed for future lab work.</li> | <li>Sterilized material and solutions needed for future lab work.</li> | ||
| - | </ol> | + | </ol> |
| - | + | ||
</p> | </p> | ||
| - | + | ||
</div> | </div> | ||
</div> | </div> | ||
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<div id="week24entry" class="nb-week"> | <div id="week24entry" class="nb-week"> | ||
<div class="twelve columns"> | <div class="twelve columns"> | ||
| - | <h3 class="centered">Week | + | <h3 class="centered">Week 24</h3> |
| - | <h5 class="centered">( | + | <h5 class="centered">(09/06 - 15/06)</h5> |
</p> | </p> | ||
| + | <div class="entry nb-wet"> | ||
| + | <div> | ||
| + | <h6>June 10th | ||
| + | <div class="nb-onetech-i nohilite">show technical details</div> | ||
| + | </h6> | ||
| + | <div class="nb-tech">Transformation was done by heat-shock. A plasmid containing ampicillin resistance was used, and the transformed cells were incubated overnight on LB plates with ampicillin. Plates showed a bacterial blanket the next day; the cells were apparently super competent.</div> | ||
| + | <p> Test of transformation efficiency of competent E. coli DH5α from June 6th. | ||
| - | + | </p> | |
| + | </div> | ||
| + | </div> | ||
Revision as of 13:37, 6 August 2014
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Team:Cornell/notebook
From 2013.igem.org
Notebook
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Wet Lab
Dry Lab
Human Practices
Wiki
Presenters
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Week 23
(02/06 - 08/06)
June 3rd
show technical details
testtesttesttest test
- Prepared SOC and yB solutions for future lab work.
- Sterilized material and solutions needed for future lab work.
June 4th
show technical details
{{{tech}}}
Made LB plates with ampicillin and ampicillin + kanamycin.
June 5th
show technical details
{{{tech}}}
Inoculated E. coli DH5α in SOC medium overnight.
June 6th
show technical details
OD of culture was 0.3160 after 100 minutes.
Made competent E. coli DH5α cells.
Week 24
(09/06 - 15/06)
June 10th
show technical details
Transformation was done by heat-shock. A plasmid containing ampicillin resistance was used, and the transformed cells were incubated overnight on LB plates with ampicillin. Plates showed a bacterial blanket the next day; the cells were apparently super competent.
Test of transformation efficiency of competent E. coli DH5α from June 6th.
