Team:Paris Saclay/Notebook/August/4

From 2014.igem.org

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(→Monday 4th August)
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The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation.  
The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation.  
 +
==== Polymerase chain reaction ====
 +
''by Sean & Pierre''
 +
 +
 +
 +
BBa_K517003
 +
 +
{| class="wikitable centre" width="50%"
 +
|+
 +
|-
 +
! scope=col | component
 +
! scope=col | volume
 +
|-
 +
|H<sub>2</sub>O
 +
|35.5μl
 +
|-
 +
| Phusion buffer 5X
 +
| 10μl
 +
|-
 +
| dNTPs
 +
| 2μl
 +
|-
 +
| iPS68bis
 +
| 1μl
 +
|-
 +
| iPS69
 +
| 1μl
 +
|-
 +
| DNA
 +
| 1μl
 +
|-
 +
| Phusion enzyme
 +
| 0.5μl
 +
|}
 +
 +
 +
BBa_K762100
 +
 +
For this BioBrick two tubes were prepared: one with undiluted DNA and one with DNA diluted 10<sup>-1</sup>.
 +
 +
{| class="wikitable centre" width="50%"
 +
|+
 +
|-
 +
! scope=col | component
 +
! scope=col | volume
 +
|-
 +
|H<sub>2</sub>O
 +
|35.5μl
 +
|-
 +
|Phusion buffer 5X
 +
|10μl
 +
|-
 +
|dNTPs
 +
|2μl
 +
|-
 +
|iPS66
 +
|1μl
 +
|-
 +
|iPS67
 +
|1μl
 +
|-
 +
|DNA
 +
|1μl
 +
|-
 +
|Phusion enzyme
 +
|0.5μl
 +
|}
'''Members there''':
'''Members there''':

Revision as of 13:50, 4 August 2014

Contents

Monday 4th August

Lab Work

A - The frame coli Odor free

Striate on Dishes

by Juliette & Romain

We striated MG1655 and MG1655Z1 on LB and Kan dishes, from the striates prepared on LB dishes 1st August

C - Salicylate Inducible Suppressing System

Plasmid DNA Purification

by Eugene and Fabio

  • Ligation of BBa_J61051 and BBa_K228001 made the 30th July

From Liquid Culture made the 1st August

Digestion to check

In progress

Electrophoresis

In progress

D - Lemon scent

Gel electrophoresis of BBa_K762100

by Sean

Samples used were prepared on the 30th July.

Results

From left to right: 5 µl of BBa_K762100 + "old" Phusion enzyme, 5 µl of BBa_K762100 + "new" Phusion enzyme

Electroporation

by Terry

Strain used: DY330 containing plasmid pJBEI6409.

We had to switch the Limonen Synthase cassette with the Apramycin resistance.

The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation.

Polymerase chain reaction

by Sean & Pierre


BBa_K517003

component volume
H2O 35.5μl
Phusion buffer 5X 10μl
dNTPs 2μl
iPS68bis 1μl
iPS69 1μl
DNA 1μl
Phusion enzyme 0.5μl


BBa_K762100

For this BioBrick two tubes were prepared: one with undiluted DNA and one with DNA diluted 10-1.

component volume
H2O 35.5μl
Phusion buffer 5X 10μl
dNTPs 2μl
iPS66 1μl
iPS67 1μl
DNA 1μl
Phusion enzyme 0.5μl

Members there:

  • Instructors and advisors: Alice, Solenne and Sylvie.
  • Students: Eugene, Fabio, Hoang Vu, Juliette, Pierre, Romain, Sean and Terry.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/August/4"