Team:Paris Saclay/Notebook/August/4

From 2014.igem.org

(Difference between revisions)
(Electroporation)
(Electroporation)
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''by Terry''
''by Terry''
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Strain used: DY330 with plasmid pJBEI6409  
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Strain used: DY330 with plasmid pJBEI6409.
The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation.  
The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation.  
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Revision as of 10:02, 4 August 2014

Contents

Monday 4th August

Lab Work

A - The frame coli Odor free

Striate on Dishes

by Juliette & Romain

We striated MG1655 and MG1655Z1 on LB and Kan dishes, from the striates prepared on LB dishes 1st August

D - Lemon scent

Gel electrophoresis of BBa_K762100

by Sean

Samples used were prepared on the 30th July.

Results

From left to right: 5 µl of BBa_K762100 + "old" Phusion enzyme, 5 µl of BBa_K762100 + "new" Phusion enzyme

Electroporation

by Terry

Strain used: DY330 with plasmid pJBEI6409. The culture has been placed at 42°C for 17 min then cooled 10 min before centrifugation.

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