Team:Paris Saclay/Protocols/PCR clean-up

From 2014.igem.org

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(→PCR clean-up)
(→PCR clean-up)
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= '''PCR clean-up''' =
= '''PCR clean-up''' =
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After the PCR, pool your PCR result in an eppendorf 1,5ml.
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# After the PCR, pool your PCR result in an eppendorf 1,5ml.
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Add 200µl NTI.
+
# Add 200µl NTI.
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+
# Centrifuge at 11000g, 30 secondes RT.
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Centrifuge at 11000g, 30 secondes RT.
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# Discard the supernatant, wash first time with 700µl NT3.
-
 
+
# Centrifuge at 11000g, 30 secondes RT.
-
Discard the supernatant, wash first time with 700µl NT3.
+
# Discard the supernatant, wash a second time with 700µl NT3.
-
 
+
# Centrifuge at 11000g, 30 secondes RT.
-
Centrifuge at 11000g, 30 secondes RT.
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# Centrifuge the column empty.
-
 
+
# Add 20µl MilliQ water and centrifuge at 11000g, 1 min RT.
-
Discard the supernatant, wash a second time with 700µl NT3.
+
# Collect your DNA in an eppendorf 1,5ml.
-
 
+
-
Centrifuge at 11000g, 30 secondes RT.
+
-
 
+
-
Centrifuge the column empty.
+
-
 
+
-
Add 20µl MilliQ water and centrifuge at 11000g, 1 min RT.
+
-
 
+
-
Collect your DNA in an eppendorf 1,5ml.
+
[https://2014.igem.org/Team:Paris_Saclay/Protocols Back to the protocols]
[https://2014.igem.org/Team:Paris_Saclay/Protocols Back to the protocols]

Revision as of 09:39, 4 August 2014

PCR clean-up

  1. After the PCR, pool your PCR result in an eppendorf 1,5ml.
  2. Add 200µl NTI.
  3. Centrifuge at 11000g, 30 secondes RT.
  4. Discard the supernatant, wash first time with 700µl NT3.
  5. Centrifuge at 11000g, 30 secondes RT.
  6. Discard the supernatant, wash a second time with 700µl NT3.
  7. Centrifuge at 11000g, 30 secondes RT.
  8. Centrifuge the column empty.
  9. Add 20µl MilliQ water and centrifuge at 11000g, 1 min RT.
  10. Collect your DNA in an eppendorf 1,5ml.

Back to the protocols

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_clean-up"