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Team:ULB-Brussels/Project/Methods
From 2014.igem.org
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<p>First, birth and growing of bacteria populations (Centrifugation, Dilution).</p> | <p>First, birth and growing of bacteria populations (Centrifugation, Dilution).</p> | ||
| - | <p>Secondly, insertion of the biobricks and plasmids chosen with E. Coli (Electroporation method, PCR Amplification, Electrophoresis), | + | <p>Secondly, insertion of the biobricks and plasmids chosen with E. Coli (Electroporation method, PCR Amplification, Electrophoresis), |
bacteria selection (in function of the quantities od oligopeptids or phoA+prolin), inclusion of a toxin-antitoxin (TA: ccdB-ccdA) system in E. Coli.</p> | bacteria selection (in function of the quantities od oligopeptids or phoA+prolin), inclusion of a toxin-antitoxin (TA: ccdB-ccdA) system in E. Coli.</p> | ||
| - | <p>Just after this step, addiction of fluorescent proteins (GFP, RFP) and determination of the quantities and properties of our bacteria | + | <p>Just after this step, addiction of fluorescent proteins (GFP, RFP) and determination of the quantities and properties of our bacteria |
| + | ---- | ||
(including Emission Spectroscopy) + analize of their genetical sequences. | (including Emission Spectroscopy) + analize of their genetical sequences. | ||
Revision as of 16:12, 3 August 2014
$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}} \newcommand{\Stabi}{\small Stabi}$ $\newcommand{\EColi}{\small E.coli} \newcommand{\SCere}{\small S.cerevisae}\\[0cm] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\PI}{\small PI}$ $\newcommand{\Igo}{\Large\mathcal{I}} \newcommand{\Tgo}{\Large\mathcal{T}} \newcommand{\Ogo}{\Large\mathcal{O}} ~$
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Abc .
First, birth and growing of bacteria populations (Centrifugation, Dilution). Secondly, insertion of the biobricks and plasmids chosen with E. Coli (Electroporation method, PCR Amplification, Electrophoresis), bacteria selection (in function of the quantities od oligopeptids or phoA+prolin), inclusion of a toxin-antitoxin (TA: ccdB-ccdA) system in E. Coli. Just after this step, addiction of fluorescent proteins (GFP, RFP) and determination of the quantities and properties of our bacteria ---- (including Emission Spectroscopy) + analize of their genetical sequences. Finally, conservation of bacteria populations that product the desired molecules in good quantities, at cold temperature in containers. | ||
- Université Libre de Bruxelles -
